Simultaneous determination of individual isothiocyanates in plant samples by HPLC-DAD-MS following SPE and derivatization with N-acetyl-l-cysteine - Publication - Bridge of Knowledge

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Simultaneous determination of individual isothiocyanates in plant samples by HPLC-DAD-MS following SPE and derivatization with N-acetyl-l-cysteine

Abstract

The procedure for the isothiocyanates (ITCs) determination that involves derivatization with N-acetyl-l-cysteine (NAC) and separation by HPLC was developed. Prior to derivatization, plant ITCs were isolated and purified using solid-phase extraction (SPE). The optimum conditions of derivatization are: 500 μL of isopropanolic eluate obtained by SPE combined with 500 μL of derivatizing reagent (0.2 M NAC and 0.2 M NaHCO3 in water) and reaction time of 1 h at 50 °C. The formed dithiocarbamates are directly analyzed by HPLC coupled with diode array detector and mass spectrometer if required. The method was validated for nine common natural ITCs. Calibration curves were linear (R2 ⩾ 0.991) within a wide range of concentrations and limits of detection were below 4.9 nmol/mL. The recoveries were in the range of 83.3–103.7%, with relative standard deviations <5.4%. The developed method has been successfully applied to determine ITCs in broccoli, white cabbage, garden cress, radish, horseradish and papaya.

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Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
FOOD CHEMISTRY no. 214, pages 587 - 596,
ISSN: 0308-8146
Language:
English
Publication year:
2017
Bibliographic description:
Pilipczuk T., Kusznierewicz B., Chmiel T., Przychodzeń W., Bartoszek-Pączkowska A.: Simultaneous determination of individual isothiocyanates in plant samples by HPLC-DAD-MS following SPE and derivatization with N-acetyl-l-cysteine// FOOD CHEMISTRY. -Vol. 214, (2017), s.587-596
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.foodchem.2016.07.125
Verified by:
Gdańsk University of Technology

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