Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour

Monika Kukowska; Michał Pikuła; Magdalena Kukowska-Kaszuba; Adriana Schumacher; Krystyna Dzierzbicka; Piotr Trzonkowski

doi:10.1039/c6ra24424f

Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour

Abstract

Several Gly-His-Lys analogues were obtained to investigate their antimicrobial properties. Three lipophilic analogues with the structure numbering 1b, 2b, 4b, exhibit significant effect against bacteria and were selected for in vitro evaluation of skin cells behaviour. In the present studies, an in vitro model of wound repair, proliferative cell staining, and tracking of living cells were used. Cell proliferation and cell migration, as important actions during the wound repair process, were studied. All the analogues provided an optimal environment for the growth, development and viability of skin cells. Microscopic changes in the morphology of fibroblasts and keratinocytes confirmed the positive effect of all the analogues on their growth and condition. Franz-type diffusion studies and the behaviour of analogues in the in vitro model of permeation were also performed. Permeability and diffusion coefficients were measured using UV-Vis spectrophotometry and RP-HPLC techniques. A substantial increase of permeability parameters for the lipophilic analogues was noticed. The outcomes of these studies suggest that lipophilic analogues possess a high potential as topical therapeutics. In relation with their proved antimicrobial activity, their safety profile and great tolerance for human skin show that they may be applied in both skin care and wound therapies.

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Authors (6)

Monika Kukowska
Michał Pikuła
- GUMed Katedra Immunologii Klinicznej i Transplantologii
Magdalena Kukowska-Kaszuba
- GuMed .
Adriana Schumacher mgr
- . atedra Immunologii Klinicznej i Transplantologii
Krystyna Dzierzbicka prof. dr hab. inż.
Piotr Trzonkowski
- . Katedra Immunologii Klinicznej i Transplantologii

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: RSC Advances no. 6, pages 1 - 11,
ISSN: 2046-2069
Language:: English
Publication year:: 2016
Bibliographic description:: Kukowska M., Pikuła M., Kukowska-Kaszuba M., Schumacher A., Dzierzbicka K., Trzonkowski P.: Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour// RSC Advances. -Vol. 6, nr. 116 (2016), s.1-11
DOI:: Digital Object Identifier (open in new tab) 10.1039/c6ra24424f
Verified by:: Gdańsk University of Technology