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The influence of pre- and postharvest treatments on selected biological and epigenetic activities of Brassica sprouts
Abstract
Introduction The Brassica vegetables are rich in glucosinolates (GLs), which are enzymatically hydrolysed to bioactive isothiocynates (ITC) and indoles, regarded as the most promising chemopreventing agents. However, in the majority of brassica sprouts the GL conversion into these beneficial derivatives occurs at the level of about 1 %, while in cabbage leaves exceeds 70%. Since sprouts represent plant material, which is frequently used for production of dietary supplements and functional foods, it is important to recognize their biological activity in the context of chemical composition. Materials and Method Sprouts were grown in the phytotrone without and with selenium addition (preharvest treatment). After harvest, a portion of sprouts was exposed on physico-chemical treatment (postharvest treatment). Biological evaluation was carried out with the aid of HT29 cell line for water extracts from lyophilized sprouts. For concentration causing less then 50% cytotoxicity, the ability to induce cytoprotective enzymes, i.e. glutathione-S-transferases (GST) and NAD(P)H:quinone oxidoreductase (NQO1), was examined. Furthermore, the influence of extracts on HDAC (class I and II) and sirtuin activity, which are a part of epigenetic regulatory mechanisms, was determined by plate-format tests offered by Promega company.
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- Category:
- Other
- Type:
- supllement, wydanie specjalne, dodatek
- Published in:
-
EUROPEAN JOURNAL OF CANCER
no. 50,
edition suppl. 5,
pages 140 - 140,
ISSN: 0959-8049 - Title of issue:
- European Journal of Cancer strony 140 - 140
- Language:
- English
- Publication year:
- 2014
- Verified by:
- Gdańsk University of Technology
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