The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge

Slawomir Ciesielski; Krzysztof Czerwionka; Dominika Sobotka; Tomasz Dulski; Jacek Mąkinia

doi:10.1007/s13353-017-0418-1

The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge

Abstract

A metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was similar to Candidatus Jettenia caeni (12.63 vs. 11.68%). This biomass was typical in terms of microbial nitrogen conversion; both ammonia (Nitrosomonas sp.) and nitrite (Nitrospira sp.) oxidizing bacteria were detected. In the lab-scale reactor, Candidatus Kuenenia stuttgartiensis and Candidatus Jettenia caeni bacteria were also present in equal proportions (18.57 vs. 20.89%). On the contrary, Candidatus Nitrospira defluvii bacteria were highly abundant in this reactor, but no known ammonia-oxidizing bacteria were detected. In light of recent studies showing that Nitrospira sp. are capable of complete nitrification, the results presented here may well indicate that both stages of nitrification in the anammox-based granular sludge reactor were performed by this bacteria.

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Authors (5)

Slawomir Ciesielski
- Uniwersytet Warmińsko-Mazurski, Wydział Nauk o Środowisku Katedra Biotechnologii w Ochronie Środowiska
Krzysztof Czerwionka dr hab. inż.
Dominika Sobotka dr inż.
Tomasz Dulski
- Uniwersytet Warmińsko-Mazurski, Wydział Nauk o Środowisku Katedra Biotechnologii w Ochronie Środowiska
Jacek Mąkinia prof. dr hab. inż.

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DOI:: Digital Object Identifier (open in new tab) 10.1007/s13353-017-0418-1
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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF APPLIED GENETICS no. 59, pages 109 - 117,
ISSN: 1234-1983
Language:: English
Publication year:: 2018
Bibliographic description:: Ciesielski S., Czerwionka K., Sobotka D., Dulski T., Mąkinia J.: The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge// JOURNAL OF APPLIED GENETICS. -Vol. 59, nr. 1 (2018), s.109-117
DOI:: Digital Object Identifier (open in new tab) 10.1007/s13353-017-0418-1
Verified by:: Gdańsk University of Technology