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The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil
Abstract
Β-asarone is a phenylpropane derivative present in the rhizomes of Acorus calamus, that was proved to exhibit toxic effects in humans. Because of its presence the whole plant that is commonly used in traditional medicine for its sedative, anti-inflammatory, neuroprotective and other properties has limited application nowadays. In the study, qualitative and quantitative analysis of a collection of nine essential oil (EO) samples of European and Asian origin was performed. The final content of β-asarone in the tested samples ranged between 0.265 and 1.885 mg/mL. Having in mind a possible application of the EO as a biopesticide, this research aimed at the development of CPC-based purification protocol that could help remove β-asarone from EO. It was proved that the biphasic solvent system composed of n-hexane/EtOAc/MeOH/water, 9:1:9:1 (v/v/v/v) was capable of the removal of the toxic constituent in the CPC chromatograph operated in the ascending elution mode with 2200 rpm and a flow rate of 5 mL/min. The chromatographic analysis that lasted only 144 min effectively separated β-asarone (purity of 95.5%) and α-asarone (purity of 93.7%) directly from the crude Acorus calamus rhizome EO.
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Authors (11)
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Mateusz Wróblewski
- Uniwersytet Łódzki
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Anna Samulewicz
- Uniwersyteckie Katolickie Liceum Ogólnokształcące w Tczewie
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Łukasz Sobiech
- Uniwersytet Przyrodniczy w Poznaniu
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Justyna Polit
- Uniwersytet Łódzki
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Wirginia Kukula-Koch
- Uniwersytet Medyczny w Lublinie
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-022-26726-6
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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Scientific Reports
no. 12,
ISSN: 2045-2322 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Szczeblewski P., Wróblewski M., Borzyszkowska-Bukowska J., Bairamova T., Górska J., Laskowski T., Samulewicz A., Kosno M., Sobiech Ł., Polit J., Kukula-Koch W.: The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil// Scientific Reports -Vol. 12,iss. 1 (2022), s.22217-
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-022-26726-6
- Sources of funding:
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- IDUB
- Verified by:
- Gdańsk University of Technology
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