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Uncertainty of antioxidant profiling in complex mixtures using liquid chromatography involving post-column derivatisation
Abstract
The main goal of this paper is to discuss the problems associated with antioxidant profiling in complex samples using a high-throughput HPLC system coupled with post-column derivatisation reactor. Based on the experimental data reported in the literature, we demonstrated that improper optimisation of temperature and/or pH assay conditions performed using an on-line derivatisation reactor may substantially change the antioxidant peaks ratio of targeted phytochemical compounds. It has been found that despite the relatively high stability of flavonoids at high temperature and under binary mobile phase HPLC conditions, the reaction of target compounds with common ABTS, FCR and DPPH radicals at elevated regions (particularly above 100 °C) dramatically changes their antioxidant activity values expressed, for example, as TEAC (Trolox equivalent antioxidant capacity) parameters. In principle, separation and detection processes of antioxidant profiling assay must not significantly affect the antioxidant activity of target compounds. In the case of foods eaten by humans or animals, critical experimental parameters such as pH and temperature concerning interaction of target analytes with derivatisation reagent should be as close as possible to the real physiological values.
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Authors (3)
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Bronisław K. Głód
- Uniwersytet Przyrodniczo-Humanistyczny Zakład Chemii Analitycznej
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Paweł Zarzycki
- Zakład Toksykologii i Bioanalityki, Wydziału Inżynierii Lądowej, Środowiska i Geodezji Politechnika Koszalińska
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
no. 33,
edition 2,
pages 216 - 219,
ISSN: 0889-1575 - Language:
- English
- Publication year:
- 2014
- Bibliographic description:
- Głód B., Kamiński M., Zarzycki P.: Uncertainty of antioxidant profiling in complex mixtures using liquid chromatography involving post-column derivatisation// JOURNAL OF FOOD COMPOSITION AND ANALYSIS. -Vol. 33, iss. 2 (2014), s.216-219
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.jfca.2013.02.012
- Verified by:
- Gdańsk University of Technology
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