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Search results for: heat shock response
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublicationEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Contribution of small heat shock proteins to muscle development and function
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Evolution towards simplicity in bacterial small heat shock protein system
PublicationEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either two...
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Numerical determination of heat exchanger response
PublicationPrzedstawiono metodę przybliżonego obliczania temperatury płynu w ustalonym punkcie wymiennika ciepła i w ustalonej chwili. Wykorzystując nieklasyczny rachunek operatorów można wyznaczyć wzór na odpowiedź wymiennika ciepła, w którym występuje m.in. całka funkcji jednej zmiennej. Do obliczenia wartości całki dla ustalonych parametrów zastosowano wzory Newtona - Cotesa. Podano również oszacowanie błędu przybliżonego obliczania wartości...
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublicationHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Impact of heat shock transcription factor 1 on global gene expression profiles in cells which induce either cytoprotective or pro-apoptotic response following hyperthermia
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Cloning and characterization of the dnaK heat shock operon of the marine bacterium Vibrio harveyi
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Pro-death signaling of cytoprotective heat shock factor 1: upregulation of NOXA leading to apoptosis in heat-sensitive cells
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Two bacterial small heat shock proteins, IbpA and IbpB, form a functional heterodimer
PublicationSmall heat shock proteins (sHsps) are a conserved class of ATP-independent chaperones which in stress conditions bind to unfolded protein substrates and prevent their irreversible aggregation. Substrates trapped in sHsps-containing aggregates are efficiently refolded into native structures by ATP-dependent Hsp70 and Hsp100 chaperones. Most γ-proteobacteria possess a single sHsp (IbpA), while in a subset of Enterobacterales, as...
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The Major Heat Shock Proteins, Hsp70 and Hsp90, in 2-Methoxyestradiol-Mediated Osteosarcoma Cell Death Model
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Mycobacterium tuberculosis Complex and Mycobacterial Heat Shock Proteins in Lymph Node Tissue from Patients with Pulmonary Sarcoidosis
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Fabrication of Electrochemical Biosensor Based on Titanium Dioxide Nanotubes and Silver Nanoparticles for Heat Shock Protein 70 Detection
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Interactive effects of vanadium and phosphorus on their uptake, growth and heat shock proteins in chickpea genotypes under hydroponic conditions
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Geldanamycin-Induced Osteosarcoma Cell Death Is Associated with Hyperacetylation and Loss of Mitochondrial Pool of Heat Shock Protein 60 (Hsp60)
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Heat shock proteins (HSP 90, 70, 60, and 27) in Galleria mellonella (Lepidoptera) hemolymph are affected by infection with Conidiobolus coronatus (Entomophthorales)
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The model homologue of the partially defective human 5,10-methylenetetrahydrofolate reductase, considered as a risk factor for stroke due to increased homocysteine level, can be protected and reactivated by heat shock proteins
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Karaś P., Kochanowicz K., Pitek M., Domański P., Obuchowski I., Tomiczek B., Liberek K.: Evolution towards simplicity in bacterial small heat shock protein system// eLife -, (2023), s.1-21
PublicationEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either...
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Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublicationWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
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Self-refraction of acoustic pulses with shock fronts in some nonequilibrium media
PublicationThe nonlinear self-refraction of acoustic pulsed beams, which include shock fronts, is studied. The medium of sound propagation is a gas where thermodynamically nonequilibrium processes take place, such as exothermic chemical reaction or excitation of vibrational degrees of a molecule’s freedom. Comparative analysis of the features of sound propagation over gases where pure nonlinear attenuation of the shock wave occurs, and gases...
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Thermal Self-Action of Acoustic Beams Containing Several Shock Fronts
PublicationThermal self-action of an acoustic beam with one discontinuity or several shock fronts is studied in a Newtonian fluid. The stationary self-action of a single sawtooth wave with discontinuity (or some integer number of these waves), symmetric or asymmetric, is considered in the cases of self-focusing and self- defocusing media. The results are compared with the non-stationary thermal self-action of the periodic sound. Thermal self-action...
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LabVIEW-based intelligent system of protection against electric shock for photovoltaic installations
PublicationEvaluation of shock hazard in installations with photovoltaic sources of energy is more complicated than in a conventional low voltage system. In case of earth fault in photovoltaic generators, that are connected to the AC power public grid, a composite waveform of earth fault current in the installation may occur. For composite waveforms, the safety criteria should be modified. The paper considers the effect of the composite waveforms...
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A method to monitor urinary carbon dioxide in patients with septic shock
PublicationSevere sepsis and septic shock are life-threatening conditions with mortality rates exceeding 31% (Levy et al., 2012) [1]. Septicemia was the most expensive US hospital condition in 2011 (Torio and Andrews, 2006)[2]. Urinary carbon dioxide may provide rapid, clinically useful information about a patient’s status, empowering physicians to intervene earlier and improve septic shock mortality. The objective of this paper is to validate...
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Influence of type of material on performance of hydraulic components in thermal shock conditions
PublicationDuring the start-up of a hydraulic system in low ambient temperatures an incorrect operation may occur. The principles and conditions of safely operating hydraulic driven machines and devices are essential to designers and operators. For this reason the author of this article has conducted a series of tests on hydraulic components and systems in thermal shock conditions (cooled-down components were supplied with hot working medium)....
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Analysis of the Heating Process of Hydraulic Motors during Start-Up in Thermal Shock Conditions
PublicationConditions that prevail during harsh winters and hot summers pose a serious challenge for machine designers building devices suitable for operation in extreme weather. It is essential for the designers and the users to define the principles and conditions for the safe operation of machines and devices with hydraulic drive in low ambient temperatures. Bearing in mind the above, the author tested the hydraulic motors in thermal shock...
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PROBLEMS OF THE STARTING AND OPERATING OF HYDRAULIC COMPONENTS AND SYSTEMS IN LOW AMBIENT TEMPERATURE (PART V) METHODS ENSURING CORRECT START-UP OF HYDRAULIC COMPONENTS OF SHIP’S ONBOARD DEVICES IN LOW AMBIENT TEMPERATURES
PublicationA large number of hydraulic devices and systems are started in low ambient temperatures. A good example of such a device is the hoisting winch on the ship. Starting hydraulic drive units in thermal shock conditions (rapid supply of hot oil to the cold unit) may lead to incorrect operation of the actuating system, for instance, due to the loss of clearance between cooperating elements. The article presents methods to prevent the...
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NON-STATIONARY THERMAL SELF-ACTION OF ACOUSTIC BEAMS CONTAINING SHOCK FRONTS IN THERMOCONDUCTING FLUID
PublicationNon-stationary thermal self-action of a periodic or impulse acoustic beam containing shock fronts in a thermoconducting Newtonian fluid is studied. Self-focusing of a saw-tooth periodic and impulse sound is considered, as well as that of a solitary shock wave which propagates with the linear sound speed. The governing equations of the beam radius are derived. Numerical simulations reveal that the thermal conductivity weakens the...
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ENHANCED EVAPORATION OF THE DROPLETS OF CONDENSATE IN THE ASYMMETRICAL SHOCK WAVE ZONE
PublicationIn the low-pressure part of steam turbine, the state path usually crosses the saturation line in penultimate stages [4,5]. The formation and evolution of these droplets lower the performance of the wet stages of the turbines, and their effects on the efficiency are collectively known as wetness losses [4]. Nowadays, due to work of steam turbines at partial load, process of homogeneous and heterogeneous condensation still is current....
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Expert opinion on the effectiveness of protection against electric shock in case of fault in an installation for electric vehicles charging
PublicationThe opinion concerns the use of residual current devices for protection against electric shock by automatic disconnection of supply in a Norwegian installation intended for charging of electric vehicles.
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PROBLEMS OF THE STARTING AND OPERATING OF HYDRAULIC COMPONENTS AND SYSTEMS IN LOW AMBIENT TEMPERATURE (PART IV) MODELLING THE HEATING PROCESS AND DETERMINING THE SERVICEABILITY OF HYDRAULIC COMPONENTS DURING THE STARTING-UP IN LOW AMBIENT TEMPERATURE
PublicationDesigners of hydraulically driven machines and devices are obliged to ensure during design process their high service life with taking into account their operational conditions. Some of the machines may be started in low ambient temperature and even in thermal shock conditions (due to delivering hot working medium to cold components). In order to put such devices into operation appropriate investigations, including experimental...
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System of protection against electric shock for circuits with power electronics converters
PublicationModern low voltage circuits are very often equipped with power electronics converters, therefore in these circuits non-sinusoidal earth currents (touch currents) may occur. For non-sinusoidal currents safety criteria should be modified. This paper presents these modified criteria and a computer system of protection against electric shock which can be implemented in circuits with power electronics converters. The system is based...
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Metrological analysis of an computerized system of protection against electric shock in circuits with variable speed drives
PublicationDrive systems, that can be operated at variable speed, are equipped with power electronics converters. This causes distortion of the earth current, and consequently the need to take into account the use of proper protective devices in relation to earth current harmonics. This paper presents a system for protection against electric shock in circuits with power electronics converters and metrological analysis indicating the requirements...
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Computer System for Evaluation of Shock Hazard in Circuits with Non-sinusoidal Earth Currents
PublicationEffects of current on people are mainly considered for 50/60 Hz sinusoidal current and smooth direct current. However, modern low voltage circuits are very often equipped with power electronics converters – rectifiers, frequency converters, therefore non-sinusoidal earth currents (touch currents) occur. For non-sinusoidal currents safety criteria should be modified. This paper presents a computer system, based on LabVIEW environment,...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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Induced Sheath Voltages In 110 Kv Power Cables – Case Study
PublicationThe paper considers shock hazard due to induced sheath voltages in 110 kV power cables. Various configurations of the metallic sheaths bonding and earthing (single-point bonding, both-ends bonding, cross-bonding) are analysed. Taking into account shock hazard and ability of the system to power transfer an optimal configuration is proposed.
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Effect of Thermal Shock During Legionella Bacteria Removal on the Corrosion Properties of Zinc-Coated Steel Pipes
PublicationThe purpose of this investigation was to conduct the failure analysis of a water-supply system made from zinc-coated steel. The observed corrosion process had an intense and complex character. The brownish deposits and perforations were present after 2-3 years of exploitation. The electrochemical study based on the Tafel polarization, corrosion potential monitoring, and electrochemical impedance spectroscopy together with microscopic...
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Funkcjonowanie zespołów napędu hydraulicznego maszyn w niskich temperaturach otoczenia
PublicationW pracy przedstawiono cztery warianty klasyfikacji rozruchów oziębionego napędu hydraulicznego. Pierwsza sytuacja dotyczy zagadnienia, w którym wszystkie zespoły układu napędowego wraz z olejem mają taką samą niską temperaturę podczas rozruchu. Pozostałe warianty odnoszą się do rozruchu w warunkach szoku termicznego wywołanych zasilaniem ciepłym czynnikiem roboczym oziębionych zespołów hydraulicznych. Rozruch zespołów...
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Induced sheath voltages in 110 kV power cables – case study
PublicationThis paper considers electric shock hazard due to induced sheath voltages in 110 kV power cables. The purpose of this paper is to find an optimal configuration of the power cable system, taking into account electric shock hazard and ability of the system to transfer maximal power. A computer simulations on a computer model of the local power system, comprising high voltage power cables, were carried out. This model enables to analyse...
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An asymmetrical λ-foot of condensing steam flow in the IMP PAN nozzle
PublicationIn the present paper we have focused on the precise prediction of the spontaneous condensation phenomena in wet steam flow. Novelty of our approach lies on modelling both the moment of initiation of a phase transition, as well as the moment of its reverse progress - called here re-vaporization of the condensate phase. The practical issue is to elaborate of a model of spontaneous condensation/vaporization of water steam flow...
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Electrical safety in low-voltage DC microgrids with B-type residual current devices
PublicationResidual current devices (RCDs) are most popular devices used in low-voltage installations for protection against electric shock and fire. In cases of high risk of electric shock the application of RCDs is mandatory. Currently, the spread of local direct current (DC) microgrids is widely considered. This creates new challenges for protective systems, in particular those based on RCDs. The main purpose of the research is to test...
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Noise related to gas bottles explosions
PublicationModern industrialized world is in a never ending danger of accidents and catastrophes of technical nature. Some of them may involve fires and explosions of gas storage bottles or containers. Technical University of Gdansk, Poland performed numerous tests where bottles containing acetylene, oxygen, hydrogen, CO2, LPG, LNG or CNG were blown due to excessive temperature caused by fire. Although those experiments were not focused on...
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Compressible gas density measurement by means of Fourier analysis of interferograms
PublicationThis paper describes a method for nonintrusive compressible gas density measurement by means of automated analysis of interferograms using FFT (Fast Fourier Transform), and its implementation using DFT (Discrete Fourier Transform), that does make this measurement technique a fairly valuable and accessible experimental method. The presented approach makes it possible to use the finite fringe setting of the interferometer, thus reducing...
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The risk assessment of adverse events of nursing activities as the element of quality management in healhcare
PublicationThe purpose of the paper is to present MedCARVER+Shock method and Pareto analysis and its usability for the risk assessment of adverse events of nursing activities. 888 activities carried out by all 190 nurses working at the District Hospital X located in Poland were taken into account. During the research the qualitative approach was used. As the result sixteen groups of nursing activities causing the highest risk of adverse events...
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Experimental verification of a new method of loop resistance testing in low voltage systems with residual current devices
PublicationA periodical verification of the effectiveness of protection against electric shock shall be performed in low voltage systems. The scope of this verification includes loop impedance/resistance testing. If a residual current device is installed in a tested circuit, this testing is problematic. A residual current device trips out during the test, because of the high value of measurement current. This precludes the execution of the...
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Analiza sterowania ułamkowego PIλDμ mocą reaktora jądrowego
PublicationW artykule przedstawiono syntezę regulatora PIλDμ niecałkowitego rzędu dla potrzeb sterowania mocą reaktora jądrowego lekko wodnego określanego, jako typu PWR (Pressurized Water Reactor). W tym celu wykorzystano nieliniowy model matematyczny reaktora PWR o parametrach skupionych obejmujący procesy generacji i wymiany ciepła oraz termicznych efektów reaktywnościowych. Nastawy regulatora PIλDμ niecałkowitego rzędu dobrano w sposób...
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Rozprzestrzenianie się w podtorzu skutków katastrof kolejowych z udziałem materiałów niebezpiecznych
PublicationDuża część przewozów materiałów niebezpiecznych prowadzona jest koleją. W związku z tym bezpieczeństwo tych przewozów nabiera coraz większego znaczenia. Każda katastrofa z udziałem materiałów niebezpiecznych ma negatywny wpływ na uczestników tego zdarzenia oraz na otaczające środowisko, bowiem jej zasięg na ogół nie jest lokalny. Z tego wynika, że w przypadku zaistnienia katastrofy należy minimalizować jej skutki oraz w dalszych...
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Computer-Aided Calculations of Earth Potential Rise in High Voltage Overhead Lines
PublicationHigh voltage overhead power lines with earth wires comprise towers made of conductive material. Some towers are in locations where people can be expected to be either for a long time or for a short time but very frequently. Evaluation of shock hazard near such towers requires calculation of line-to-earth short-circuit current, earth current and especially earth potential rise. Number of towers in high voltage power lines can exceed...
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RCDs Tripping in the Range from DC to AC 50 kHz for Slowly Rising Residual Current
PublicationThe wide use of power electronic converters means that in many low-voltage systems earth fault currents containing components from DC to AC of various frequencies have to be taken into account. Due to the tendency to increase the modulation frequency in converters, components of higher frequencies may be in the order of several tens of kilohertz. Therefore, it is very important to verify the behavior of devices for protection against...
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Chemical Aspects of Biological Activity of Isothiocyanates and Indoles, the Products of Glucosinolate Decomposition
PublicationThere is growing evidence that cancer chemoprevention employing natural, bioactive compounds may halt or at least slow down the different stages of carcinogenesis. A particularly advantageous effect is attributed to derivatives of sulfur-organic phytochemicals, such as glucosinolates (GLs) synthesized mainly in Brassicaceae plant family. GLs are hydrolysed enzymatically to bioactive isothiocyanates (ITC) and indoles, which exhibit strong...
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Numerical Simulations for Transonic Flow in Control Valve
PublicationResults of numerical simulations for transonic flow in control valve are presented. The valve is the main part of an adaptive pneumatic shock absorber. Flow structure in the valve domain and the influence of the flow non-uniformity in the valve on a mass flow rate is investigated. Numerical simulation results are compared with experimental data.
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A new method of fault loop resistance measurement in low voltage systems with residual current devices
PublicationThis paper presents a new method of fault loop resistance measurement in low voltage systems with residual current devices. The method enables measuring fault loop resistance without nuisance tripping of residual current devices, by application an unconventional waveform of measurement current. It is important for proper verification of the effectiveness of protection against electric shock.