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Search results for: RPOE SIGMA FACTOR
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublicationEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublicationTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Gracjana Klein-Raina dr hab.
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...
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Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
PublicationThe outer membrane (OM) of Gram-negative bacteria is asymmetric due to the presence of lipopolysaccharide (LPS) facing the outer leaflet of the OM and phospholipids facing the periplasmic side. LPS is essential for bacterial viability, since it provides a permeability barrier and is a major virulence determinant in pathogenic bacteria. In Escherichia coli, several steps of LPS biosynthesis and assembly are regulated by the RpoE...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublicationDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublicationThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublicationEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
PublicationMass spectrometric analyses of lipopolysaccharide (LPS) from isogenic Escherichia coli strains with nonpolar mutations in the waa locus or overexpression of their cognate genes revealed that waaZ and waaS are the structural genes required for the incorporation of the third 3-deoxy-α-D-manno-oct-2-ulosonic acid (Kdo) linked to Kdo disaccharide and rhamnose, respectively. The incorporation of rhamnose requires prior sequential incorporation...
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Small regulatory bacterial RNAs regulating the envelope stress response
PublicationMost bacteria encode a large repertoire of RNA-based regulatory mechanisms. Recent discoveries have revealed that the expression of many genes is controlled by a plethora of base-pairing noncoding small regulatory RNAs (sRNAs), regulatory RNA-binding proteins and RNA-degrading enzymes. Some of these RNA-based regulated processes respond to stress conditions and are involved in the maintenance of cellular homeostasis. They achieve...
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The Implementation of the Taxonomic Spatial Measure of Development in the Analysis of Convergence in the Standard of Living
PublicationThe main goal of this paper is to analyse the existence of the social convergence in the European Union between 19952012. The social convergence refers to a reduction in the dispersion of the standard of living across countries. A taxonomic spatial measure of development was used as the standard of living approximation. The use of the new approach proposed by E. Antczak allowed for an explanation of the disparities in the analysed...
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Biofilm formation and its regulation in Staphylococcus aureus
PublicationInfections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and...
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Analog fault signature based on sigma-delta modulation and oscillation-test methodology.
PublicationW artykule dokonano przeglądu prac z zakresu testowania układów elektronicznych metodą oscylacyjną. Wskazując na niedostatki aktualnie stosowanej techniki testowania oscylacyjnego zaproponowano nową sygnaturę uszkodzeń dla układów analogowych. Proponowany parametr diagnostyczny jest wydobywany z odpowiedzi czasowej oscylatora testującego, w układzie złożonym z detektora szczytowego, modulatora sigma-delta oraz licznika rewersyjnego....
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Uwarunkowania aplikacyjne koncepcji Six Sigma w sektorze MŚP
PublicationArtykuł porusza istotne, z punktu widzenia podnoszenia konkurencyjności sektora MŚP a rzadko przedstawiane w literaturze krajowej, zagadnienie wykorzystania w tej grupie przedsiębiorstw koncepcji Six Sigma. Obok charakterystycznych cech MŚP wywierających wpływ na zastosowanie w nich metodyki Six Sigma, przedstawiono związki tej koncepcji z systemami zarządzania jakością.
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Assessment of preparedness for Lean Six Sigma projects in small and medium-sized organizations
PublicationThe article presents a proposal for the maturity indicator of Lean Six Sigma projects within the framework of the original comprehensive methodology of Lean Six Sigma implementation dedicated to the SME sector. The indicator allows an LSS project to be matched to the real needs and preparedness of any small or medium-sized organization. The indicator was designed as a result of pilot research conducted in Polish and French SMEs...
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Elements of six sigma methodoloygy in polish smes. the pomeranian region context
PublicationIn this article, it has been suggested that the effectiveness of the design and implementation of the process approach in the SMEs sector affects the effectiveness and efficiency of the application of elements of the Six Sigma ™ concept. The selected results of the research have been presented to answer the question to what extent small and medium-sized manufacturing, service and production - service companies in Pomeranian region...
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How to adapt Lean Six Sigma to improve performance of SMEs of manufacturing sector
PublicationThe main objective of the paper is to present the utilitarian effect of the adaptation of the Lean Six Sigma concept and its implementation in the small and medium-sized enterprises of the manufacturing sector. The original methodology was developed on the basis of research carried out in the Polish and French enterprises from 2009 to 2011. In the case of SMEs, the use of the concept of Lean Six Sigma is much less common, as...
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Six Sigma w małych i średnich przedsiębiorstwach. Puste hasło czy konieczność?
PublicationW artykule przedstawiono aspekty wykorzystania w sektorze małych i średnich przedsiębiorstw (MŚP) elementów koncepcji Six Sigma. Celem tego opracowania jest ocena potrzeb i uwarunkowań aplikacyjnych koncepcji Six Sigma w sektorze MŚP, zwłaszcza w nawiązaniu do bazujących na podejściu procesowym systemów zarządzania jakością. Zaprezentowano charakterystyczne cechy mniejszych organizacji, stanowiące w kontekście programów Six Sigma...
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Design for Six Sigma
e-Learning Courses -
Wsparcie informatyczne koncepcji Lean Management oraz Six Sigma
PublicationW rozdziale tym przedstawiono kluczowe aspekty integracji dwóch najważniejszych obecnie koncepcji optymalizacji procesów w organizacjach produkujących wyroby materialne oraz świadczących usługi – Six Sigma i Lean Manufacturing. Szczególną uwagę zwrócono na uwarunkowania wsparcia informatycznego tych koncepcji w mniejszych organizacjach.
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Simulation analysis of a production process with selected six sigma ratios
PublicationComputer technologies allow more and more to model as well as to perform simulation experiments of various processes. The simulation analysis provides a better understanding of the interdependencies between various stages of production processes.The results of simulation studies were presented, the aim of them was to show the opportunities of the analysis of the process according to the scenarios and variants developed in connection...
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Automatyzacja procesów pomiarowych w metodyce Six Sigma w sektorze MŚP
PublicationCelem niniejszego artykułu jest przedstawienie metrologicznych aspektówmetodyki Six Sigma w świetle nowoczesnych rozwiązań technologicznych.Pomiary są nieodłącznym elementem metodyki Six Sigma, gdyż na ich bazieprowadzi się nadzorowanie procesów produkcyjnych. Tradycyjne metody pomiarowe mogą występować w produkcji jednostkowej, natomiast produkcjaseryjna i masowa wymaga automatyzacji procesów pomiarowych.
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Assessment of the Small Enterprise’s Maturity to Improvement Projects Based on the Lean Six Sigma Concept
PublicationThis paper explores the methodology to assess the maturity of small enterprise of building masonry sector to implement integrated approach of Lean Six Sigma. For this purpose survey data from senior managers were used. Quantitative method of the case organization’s needs and capabilities in the scope of Lean Six Sigma project implementation was applied. The value of maturity indicator for Lean Six Sigma projects dedicated to the...
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Wdrażanie Lean Six Sigma w MŚP sektora produkcyjnego
PublicationW opracowaniu przedstawiono rozważania dotyczące wprowadzania metodyki Lean Six Sigma w sektorze małych i średnich przedsiębiorstw produkcyjnych. Zaprezentowano odwołującą się do kontekstu tej metodyki charakterystykę MŚP oraz zaproponowano 5 etapowy model implementacyjny uwzględniający czynniki wpływające na skuteczne i adekwatne do możliwości i potrzeb MŚP adaptowanie tego podejścia.
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HOW TO ASSESS THE MATURITY OF SMALL AND MEDIUM-SIZED ENTERPRISES TO LEAN SIX SIGMA PROJECTS
PublicationThis article describes the considerations for the implementation of the Lean Six Sigma (LSS) methodology in small and medium-sized enterprises. Pilot research was conducted in Polish and French SMEs (nine Polish and nine French enterprises from the service and production sectors) referring to the context of the implementation and application of the Lean Six Sigma concept. The studies led to the identification of the motives, needs,...
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Lean Six Sigma in French and Polish Small and Medium-Sized Enterprises - The Pilot Research Results
PublicationThe article presents results of the research conducted in Polish and French small and medium-sized enterprises referring to the context of the implementation and application of the concept of Lean Six Sigma. The research was conducted in the form of case studies, observations and individual interviews. The pilot studies led to the identification of the needs, expectations, concerns and experiences of these companies. At the same...
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Reduction of exceeding the guaranteed service time for external trucks at the DCT Gdańsk container terminal using a six sigma framework
PublicationPurpose The purpose of this research was to investigate ways to reduce the average amount of exceeded guaranteed service time for external trucks at Deepwater Container Terminal Gdańsk Sp z o.o. (DCT Gdańsk) via dosing the gate activities, in particular IN-Gate entry process of trucks carrying import/export/transit containers. Design/methodology/approach A Six Sigma methodology with the DMAIC methods along with the SIPOC chart,...
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Teoretyczne i praktyczne aspekty zastosowania modulatora sigma-delta w testerze wbudowanym BIST
PublicationPrzedstawiono teoretyczne i praktyczne aspekty zastosowania modulatora sigma-delta w testerze wbudowanym BIST, przeznaczonym do diagnostyki mieszanych sygnałowo pakietów elektronicznych. Omówiono podstawowe parametry modulatorów. Przeprowadzono dyskusję możliwych wariantów wyboru rodzaju, parametrów, architektury oraz technologii wykonania modulatora sigma-delta. Opracowano behawioralny model modulatora drugiego rzędu o własciwościach...
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Zastosowanie metody SIX SIGMA do analizy półfabrykatów łożysk ślizgowych turbin parowych
PublicationObecnie w różnych dziedzinach życia, rónież w przemyśle, zaobserwowano coraz większe zainteresowanie zagadnieniami związanymi z metodą usprawniania procesów SIX SIGMA. Podjęto decyzję, aby także podczas produkcji łożysk ślizgowych do turbin parowych przeprowadzić konstrukcyjno-technologiczną analizę półfabrykatów pod względem ich różnorodności, użytych gatunków materiału, cyklu dostawy oraz czasu związanego z dalszym przebiegiem...
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LSS Plutus - Lean Six Sigma dla małych i średnich przedsiębiorstw
PublicationKsiążka w przystępny sposób prezentuje metodykę adaptującą koncepcję Lean Six Sigma do potrzeb małych i średnich przedsiębiorstw w Polsce. Autorzy pracy zwracają jednocześnie uwagę na specyfikę tego sektora w naszym kraju, przedstawiając jednocześnie przykłady dobrych praktyk stosowanych z powodzeniem w przedsiębiorstwach francuskich.
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Characterization of small and medium enterprises (SMES) of Pomeranian Region in six sigma methodology application
PublicationThe paper presents the rarely researched and important issue for SMEs competitiveness - knowledge and practical application of Six Sigma methodology. The results of research conducted by Authors allow to answer the question - to what degree SMEs of Pomeranian region are aware of importance and prepared for systemic control and improvement of their processes?W opracowaniu poruszono istotne, z punktu widzenia podnoszenia konkurencyjności...
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World class manufacturing: integrated improvement program based on six sigma, lean and TPM methodologies
PublicationWCM jest integracją najlepszych praktyk zarządzania procesami produkcyjnymi. Jest filozofią bazującą na LEAN, SIX SIGMA oraz TPM.
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Model integracji koncepcji Lean Management i Six Sigma w sektorze MŚP
PublicationW opracowaniu przedstawiono propozycję metodyki wprowadzania i wykorzystania elementów koncepcji Lean Six Sigma w sektorze MŚP. Metodyka została poddana weryfikacji w średniej wielkości francuskiej firmie produkcyjnej. Wnioski płynace z tej weryfikacji przyczyniły się do udoskonalenia proponowanych rozwiązań
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Lean Six Sigma Black Belt
e-Learning Courses -
Forum of Mathematics Sigma
Journals -
Zarządzanie procesami z zastosowaniem metodyki Six Sigma w sektorze MŚP. Kontekst lokalny i sektorowy
PublicationW artykule postawiono tezę, że skuteczność projektowania i implementacji orientacji procesowej w sektorze MŚP decyduje o skuteczności i efektywności zastosowań elementów koncepcji Six Sigma™. Przedstawiono wybrane wyniki badań mających odpowiedzieć na pytanie - w jakim stopniu małe i średnie firmy produkcyjne, usługowe oraz produkcyjno-usługowe w regionie pomorskim są świadome znaczenia i przygotowane do systemowo ujętego nadzorowania...
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Wykorzystanie metodyki Lean Six Sigma w doskonaleniu funkcjonowania małych i średnich przedsiębiorstw
PublicationCelem pracy jest przedstawienie wyników badań oraz utylitarnych aspektów wynikających z opracowania oryginalnej metodyki wdrażania metodyki Lean Six Sigma w małych i średnich przedsiębiorstwach sektora produkcyjnego. Metodyka ta powstała na podstawie badań przeprowadzonych w polskich i francuskich przedsiębiorstwach w latach 2009-2011. W opracowaniu został zaprezentowany zarys proponowanej metodyki oraz przykład jej zastosowania...
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Uwarunkowania wykorzystania metodyki Six Sigma w sektorze MŚP z uwzględnieniem możliwości zastosowania aplikacji komputerowych
PublicationCelem niniejszego opracowania jest zaprezentowanie współczesnych rozwiązań informatycznych stosowanych w celu usprawnienia funkcjonowania metodyki Six Sigma ze szczególnym zwróceniem uwagi na ich adaptację w sektorze małych i średnich przedsiębiorstw (MŚP).
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Verification of baffle factor for straight pipe flow
PublicationDuring the water disinfection devices designing, it is often assumed that the baffle factor for a straight pipe reactors is equal to one. It would be possible only for the plug flow, which is a simplified model of the flow and does not appear in real situations. The paper contains an equation which enables calculation of the real value of the baffle factor for the pipe flow.
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Within- and between-person factor structure of the Oldenburg Burnout Inventory: Analysis of a diary study using multilevel confirmatory factor analysis
PublicationThe study examined the factor structure of burnout, as measured with the Oldenburg Burnout Inventory. The participants were 235 employees of a public administration agency who assessed their burnout online for 10 consecutive working days. Two models were tested with multilevel confirmatory factor analysis, assuming the same one or two-factor structure at the within- and between-person levels. Both models showed a reasonable fit...
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Share of the wage factor in national income – selected aspects
PublicationThe aim of this study is to examine changes in the share of the wage factor in national income, in selected EU countries against labour productivity and investment rates reflecting changes in capital-labour relations, indicated by researchers as determinants of change of the share of the wage factor in national income. The scope of the study includes a review of literature devoted to the issue of wage factor share in national income...
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How much is Impact Factor worth?
PublicationThe purpose of this work is to present an aspect of counting citations, which is correlated to the Impact Factor, very important for journals in scientific communication, and provides information about the position of a scientific journal in a particular research area. Sometimes citations don’t count in a research output, e.g. if an article is published in a predatory journal. Those journals ...
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Reduction of exceeding the guaranteed service time for external trucks at the DCT Gdańsk container terminal using a six sigma framework
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Sigma profiles of DES components
Open Research DataThe Sigma profiles for DES components created by the COSMO-RS.
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Scaling of numbers in residue arithmetic with the flexible selection of scaling factor
PublicationA scaling technique of numbers in resudue arithmetic with the flexible selection of the scaling factor is presented. The required scaling factor can be selected from the set of moduli products of the Residue Number System (RNS) base. By permutation of moduli of the number system base it is possible to create many auxilliary Mixed-Radix Systems associated with the given RNS with respect to the base, but they have different sets...
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Estimation of Rician K-factor Values in Urban Terrain
PublicationThis paper presents results of Rician K-factor estimation from CDMA2000 downlink signals recorded in the area of medium city. Signal and data processing methods used to estimate fading parameters are also briefly described.
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Image as a key factor of brand value at social media
PublicationThe paper aims to investigate the relationship between social media presence and activities and brand value for the most valuable Global Brands 2012 (Interbrandt 2013). Social media communication is an innovative way of communication. Brand value is a key performance indicator of brand strategy for managers. Brand and category image realized to be the key factor of investigated relation.
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The Influence of LED Lighting Sources on the Nature of Power Factor
PublicationThis article presents measurements of electric power absorbed by a newly built facility for office and scientific research activities. These measurements highlighted the need for compensation of capacitive reactive power—not predicted by the designer—due to the vast use of LED lighting in the facility. The article also describes a reactive power compensation system, designed on the basis of the above-mentioned measurements, and...
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Anticancer imidazoacridinone C-1311 inhibits hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and angiogenesis
PublicationAntitumor imidazoacridinone C-1311 is a DNA-reactive topoisomerase II and FLT3 receptor tyrosine kinase inhibitor. Here, we demonstrate the mechanism of C-1311 inhibitory action on novel targets: hypoxia-inducible factor-1α (HIF-1α), vascular-endothelial growth factor (VEGF), and angiogenesis. In a cell-free system, C-1311 prevented HIF-1α binding to an oligonucleotide encompassing a canonical hypoxia-responsive element (HRE),...