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Search results for: fluorescence
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JOURNAL OF FLUORESCENCE
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Initial Stages of Angiosperm Greening Monitored by Low-Temperature Fluorescence Spectra and Fluorescence Lifetimes
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Fluorescence imaging spectroscopy and microscopy
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Comprehensive Review of Fluorescence Applications in Gynecology
PublicationSince the introduction of indocyanine green (ICG) as a fluorophore in near-infrared imaging, fluorescence visualization has become an essential tool in many fields of surgery. In the field of gynecology, recent new applications have been proposed and found their place in clinical practice. Different applications in gynecology were investigated, subcategorized, and overviewed concerning surgical applications and available dyes....
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Fluorescence of p-hydroxyazobenzocrowns – Tautomeric equilibrium effect
PublicationThe spectroscopic properties of a series of para-hydroxyazobenzocrowns, including three novel compounds, were investigated using UV–Vis absorption and emission spectroscopy. This study presents, for the first time, determined quantum yield (QY) values for macrocycles of this category, ranging between 0.122 and 0.195. The highest values were obtained for crowns bearing two phenyl substituents in benzene rings. The impact of aromatic...
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Fluorescence Measurements for chemical optical sensor of cobalt
PublicationW pracy zaproponowano i opisano optoelektroniczny fluorescencyjny sensor do pomiaru stężenia kobaltu w wodzie.
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Protochlorophyll complexes with similar steady-state fluorescence characteristics can differ in fluorescence lifetimes. A model study in Triton X-100
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The Early Stages of Photosystem II Assembly Monitored by Measurements of Fluorescence Lifetime, Fluorescence Induction and Isoelectric Focusing of Chlorophyll-Proteins in Barley Etiochloroplasts
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Secondary emission influenced fluorescence decay of a homogeneous fluorophore solution
PublicationAn analytical expression is found allowing the calculation of the secondary emission influenced fluorescence decay of a homogeneous fluorophore solution. Before starting the calculation one has to know the shape of the primary fluorescence decay of the fluorophore and the value of the parameter kappa denoting the ratio of the secondary to primary steady-state fluorescence intensities. The method elaborated by Budo and Ketskemety...
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Characterization of metabolites in different kiwifruit varieties by NMR and fluorescence spectroscopy
PublicationIt is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of 1H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data...
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Flavin mononucleotide fluorescence intensity decay in concentrated aqueous solutions
PublicationCzasowo-rozdzielone widma absorpcji i fluorescencji FMN w roztworach wodnych zostały zmierzone dla szerokiego zakresu stężeń. Pokazano, że zaniki fluorescencji są silnie przyśpieszone przez obecność dimerów FMN, które stanowią niedoskonałe pułapki energii wzbudzenia. Wyniki doświadczalne porównano z rezultatami symulacji Monte-Carlo, uwzględniającej powrotny transfer energii od pułapek do donorów, stwierdzając dobrą zgodność modelu...
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Simple adaptive cubic spline interpolation of fluorescence decay functions
PublicationZaproponowano prostą metodę interpolacji funkcji zaniku fluorescencji. W pierwszym kroku interpolowany zanik jest całkowany za pomocą adaptacyjnego algorytmu wykorzystującego kwadratury Newtona-Cotesa. Uzyskiwana w ten spoób siatka wartości czasu jest używana w drugim kroku polegającym na typowej interpolacji za pomocą funkcji sklejanych trzeciego stopnia.
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The usefulness of fluorescence in detecting ischemia during bariatric and metabolic surgery
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Fluorescence emission of carbon disulfide in low energy electron collision.
PublicationZbadano widma emisyjne dwusiarczku węgla w zakresie widmowym od 235 nm do575 nm wzbudzane zderzeniami elektronów o energii 9.7eV I 15eV. W otrzymanych widmach największe natężenie miały pasma pochodzące z dysocjacji cząsteczek dwusiarczku węgla.
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Pyrrole azocrown ethers - synthesis, crystal structures, and fluorescence properties
PublicationZsyntezowano nowe związki makrocykliczne zawierające w makropierścieniu pierścień pirolowy, grupy azowe oraz reszty chromoforowe i fluorescencyjne. Scharakteryzowano strukturę krystaliczną dwóch spośród otrzymanych makrocykli metodą rentgenografii strukturalnej. Stwierdzono, że zaprezentowane pirolowe azokorony to związki czułe i selektywne na jony ołowiu(II) oraz, że mogą być użyte jako chemosensory zarówno w spektroskopii UV-Vis,...
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Electron impact fragmentation of pyrrole molecules studied by fluorescence emission spectroscopy
PublicationThe fluorescence emission spectroscopy using electron impact excitation technique was employed to study fragmentation processes of the gas phase pyrrole molecules. The following excited fragmentation species were observed by detection of their fluorescence decay: the atomic hydrogen H(n), n = 4-7 and the diatomic CH(A2Δ), CN(B2Σ+), NH(A3Π) and C2(d3Πg) fragments. These atomic and molecular products differ from those previously...
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Metal enhanced fluorescence of flavin mononucleotide using new plasmonic platform
PublicationNew plasmonic platform was successfully obtained to investigate the increase of fluorescence intensity of a fluorophore in the presence of silver nanoparticles. A flavin mononucleotide, was selected by us as a fluorophore for this study as a very important biological compound playing a key role in many biochemical process. Plasmonic platforms were characterized by means of luminescence spectroscopy. Flavin mononucleotide deposited...
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Effect of xanthophyll pigments on fluorescence of chlorophyll a in LHC II embedded to liposomes
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Electron impact fragmentation of pyrrole molecules studied by fluorescence emission spectroscopy
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Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants
PublicationThe main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants...
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Fluorescence of nanodiamond cocktails: pH-induced effects through interactions with comestible liquids
PublicationFluorescent nanodiamonds with nitrogen-vacancy centers have become important nanoscale probes for sensing and imaging. The surface chemistry of the nanodiamonds influences their emission, interactions, and quantum properties. In this work, we propose to utilize fluorescent nanodiamonds as photostable markers for investigation of comestible liquids. We prepared nanodiamond/comestibles suspensions/cocktails with a wide range of pH...
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Fluorescence Imaging Using Methylene Blue Sentinel Lymph Node Biopsy in Melanoma
PublicationIntroduction: Fluorescence imaging of sentinel node biopsy in melanoma is a novel method. Both indocyanine green (ICG) and methylene blue (MB) have fl uorescent properties. The aim of this study was to present, for the fi rst time in a clinical series of patients, the possible usage of MB as a fl uorescent dye for sentinel node biopsy during surgery for melanoma. Material and methods: Twenty patients with...
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Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector
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Fluorescence use in minimally invasive metabolic and bariatric surgery — a systematic review of the literature
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Fluorescence depolarization in the presence of excitation energy migration in partly ordered polymer films.
PublicationW pracy przedstawiono wyniki pomiarów i symulacji Monte Carlo zaników anizotropii emisji fluorescencji dla układu 3,3'-diethylthiacyanine iodine (barwnik) w osiowo rozciąganych foliach polimerowych.
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Near-Infrared Fluorescence Image-Guided Surgery in Esophageal and Gastric Cancer Operations
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Methylene Blue Near-Infrared Fluorescence Imaging in Breast Cancer Sentinel Node Biopsy
PublicationIntroduction: Fluorescence-based navigation for breast cancer sentinel node biopsy is a novel method that uses indocyanine green as a fluorophore. However, methylene blue (MB) also has some fluorescent properties. This study is the first in a clinical series presenting the possible use of MB as a fluorescent dye for the identification of sentinel nodes in breast sentinel node biopsy. Material and methods: Forty-nine patients with...
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Solvent effects on fluorescence properties of protochlorophyll and its derivatives with various porphyrin side chains
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Fluorescence Lifetimes Study of α-Tocopherol and Biological Prenylquinols in Organic Solvents and Model Membranes
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KMgF3:Eu2+ as a new fluorescence-based pressure sensor for diamond anvil cell experiments
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Tocopheryl Succinate-Induced Structural Changes in DPPC Liposomes: DSC and ANS Fluorescence Studies
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Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island film
PublicationOpisano fluorescencję barwnika CY5 połączonego z DNA w roztworze buforowym na wysepkach srebrowych.
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Concentration-Dependent Fluorescence Properties of Rhodamine 6G in Titanium Dioxide and Silicon Dioxide Nanolayers
PublicationWytworzono cienkie warstwy zawierające rodaminę 6G w dwutlenku tytanu (R6G/TiO2) i w dwutlenku krzemu (R6G/SiO2) stosując metodę zol-żel. Badane były dwa rodzaje matryc, w których domieszkowano barwnik rodamina 6G (R6G) o różnych koncentracjach. Został stwierdzony istotny wpływ koncentracji barwnika na widma absorpcji i emisji oraz na czasowo rozdzielone widma fluorescencji. W szczególności stwierdzono znacznie mniejszą zdolność...
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Ethylene decomposition on TiO2 - UV and Fluorescence lamp
Open Research DataData contain chromatograms recorded during decomposition of ethylene flowing over TiO2 through the quartz tubular shape reactor. TiO2 was coated onto glass plates with dimension of 2cmx2cm, in total 3 plates were used and placed inside reactor. Reactor was irradiated by UV (Special 'TL'E, Philips) or fluorescent (Lumilux, OSRAM) 3 ring-shaped lamps,...
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Methods and Applications in Fluorescence
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Characterization of Bioactivity of Selective Molecules in Fruit Wines by FTIR and NMR Spectroscopies, Fluorescence and Docking Calculations
PublicationFourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) spectroscopies were applied to characterize and compare the chemical shifts in the polyphenols’ regions of some fruit wines. The obtained results showed that FTIR spectra (1800–900 cm−1) and 1H NMR (δ 6.5–9.3 ppm) of different fruit wines can be used as main indices of the year of vintage and quality of fruit wines. In addition to the classical determination...
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Photofragmentation of tetrahydrofuran molecules in the vacuum-ultraviolet region via superexcited states studied by fluorescence spectroscopy
PublicationPhotofragmentation of tetrahydrofuran molecules in the vacuum-ultraviolet region, producing excited atomic and molecular fragments, has been studied over the energy range 14-68 eV using photon-induced fluorescence spectroscopy. Excited hydrogen atoms H(n), n = 3-11, have been detected by observation of the Hα to Hı lines of the Balmer series. The diatomic CH(A2Δ), CH(B2Σ-) and C2(d3Πg) fragments, which are excited to low vibrational...
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In vivo imaging of the human eye using a two-photon excited fluorescence scanning laser ophthalmoscope
PublicationBACKGROUND. Noninvasive assessment of metabolic processes that sustain regeneration of human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics and to accelerate development of new treatments to counter retinal diseases. Fluorescent vitamin A derivatives, which are the chemical intermediates of these processes, are highly sensitive to UV light; thus, safe analyses of these processes in humans...
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Photofragmentation of tetrahydrofuran molecules in the vacuum-ultraviolet region via superexcited states studied by fluorescence spectroscopy
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Assessing the Interactions of Statins with Human Adenylate Kinase Isoenzyme 1: Fluorescence and Enzyme Kinetic Studies
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Disruptive effect of tocopherol oxalate on DPPC liposome structure: DSC, SAXS, and fluorescence anisotropy studies
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Unraveling Energy Transfer and Fluorescence Quenching Dynamics in Biomolecular Complexes: A Comprehensive Study of Imiquimod-Rifampicin Interaction.
PublicationIn nature, numerous biomolecules are implicated in charge transfer (CT) and energy transfer (ET) mechanisms crucial for fundamental processes such as photosynthesis. Unveiling these mechanisms is pertinent to multiple disciplines including chemistry, engineering and biochemistry. This letter presents a study involving two molecules forming a model system with efficient ET properties. Specifically, their complex exhibits dark quenching...
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Valorization of waste cabbage leaves by postharvest photochemical treatments monitored with a non-destructive fluorescence-based sensor
PublicationThe biosynthesis of polyphenolic compounds in cabbage waste, outer green leaves of white head cabbage (Brassica oleracea L. var. capitata subvar. alba), was stimulated by postharvest irradiation with UVB lamps or sunlight. Both treatments boosted the content of kaempferol and quercetin glycosides, especially in the basal leaf zone, as determined by the HPLC analysis of leaf extracts and by a non-destructive optical sensor. The...
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Photoactive Protochlorophyllide-Enzyme Complexes Reconstituted with PORA, PORB and PORC Proteins of A. thaliana: Fluorescence and Catalytic Properties
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The processing of staphylococcal biofilm images from Fluorescence and Confocal Microscopy to assess in vitro efficacy of antiseptic molecules
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Fluorescence anisotropy decay in the presence of multistep energy migration and back transfer in disordered two-component systems
PublicationPrzeprowadzono analizę zaniku anizotropii fluorescencji w sztywnym (lepkim) układzie dwuskładnikowym donor (Rodamina 6G)- akceptor (Rodamina B) z uwzględnieniem prostego i powrotnego transferu energii. Stwierdzono bardzo dobrą zgodność danych doświadczalnych z wynikami symulacji Monte Carlo. W pracy zaprezentowano specjalnie opracowany hybrydowy algorytm symulacji Monte Carlo anizotropii emisji, który jest bardzo wydajny w porównaniu...
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O 1s excitation and ionization processes in the CO2molecule studied via detection of low-energy fluorescence emission
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Electromodulation of fluorescence in hole-transporting materials for organic light-emitting diodes. Part II: Straburst amines
PublicationPraca jest kontynuacją artykułu, który został opublikowany wChem. Phys. vol. 256 (2000) s. 351-362. Zaobserwowano silne wygaszenie fluorescencji w dendrymerach aminowych zbudowanych na bazie trifenyloaminy. Efekt ten został przypisany rozpadowi stanów emitujących światło pod działaniem silnego pola elektrycznego.
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Site-to-site distance distribution in flexible molecules: theoretical evaluation of the donor and/or acceptor fluorescence decay function
PublicationPrzedstawiono wyrażenie teoretyczne opisujące zależność funkcji zaniku fluorescencji od rozkładu odległości P(r) pomiędzy donorami i akceptorami w giętkich molekułach dwuchromoforowych. Wyrażenie uwzględnia wiekoeksponencjalny zanik izolowanych donorów i akceptorów, a także możliwość niekompletnego oznakowania molekuł akceptorami. Przyjęto, że względne zmiany odległości donorów i akceptorów w czasie życia fluorescencji donora mogą...
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Effects Induced by the Temperature and Chemical Environment on the Fluorescence of Water-Soluble Gold Nanoparticles Functionalized with a Perylene-Derivative Dye
PublicationWe developed a fluorescent molecular probe based on gold nanoparticles functionalized with N,N′-bis(2-(1-piperazino)ethyl)-3,4,9,10-perylenetetracarboxylic acid diimide dihydrochloride, and these probes exhibit potential for applications in microscopic thermometry. The intensity of fluorescence was affected by changes in temperature. Chemical environments, such as different buffers with the same pH, also resulted in different fluorescence...