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Catalog Publications

Year 2013
Year 2012
Year 2011
Year 2009
Year 2007
  • Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
    Publication
    • M. Liebscher
    • G. Jahreis
    • C. Lücke
    • S. Grabley
    • S. Raina
    • C. Schiene-Fischer

    - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2007

    We have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...

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Year 2004
Year 2003
  • Dehydration converts DsbG crystal diffraction from low to high resolution
    Publication
    • B. Heras
    • M. A. Edeling
    • K. A. Byriel
    • A. Jones
    • S. Raina
    • J. L. Martin

    - STRUCTURE - Year 2003

    Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution....

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  • Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
    Publication

    - MOLECULAR MICROBIOLOGY - Year 2003

    Escherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...

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Year 2002
  • Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
    Publication
    • C. Tam
    • B. Collinet
    • G. Lau
    • S. Raina
    • D. Missiakas

    - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2002

    Eo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...

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