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The electrochemical response to Au electrode modification steps towards viral electrochemical immunosensor preparation
Opis
The dataset contains the electrochemical measurements (CV/EIS) carried out for the consecutive steps of the Au electrode functionalization, in separate subfolders, as described. The cyclic voltammetry (CV) was conducted using a Palmsens 4 potentiostat/galvanostat system (Methrom, Autolab, Netherlands) in the standard three-electrode configuration, with the Pt mesh was used as a counter electrode, and Ag/AgCl/0.1M KCl as a reference electrode. In the case of the EIS, the frequency ranged from 10 kHz to 0.2 Hz for Au electrode with 40 points. The amplitude of the alternating current (AC) signal was 10 mV. Each potential was held constant for 5 s before each measurement to obtain steady-state conditions. All electrochemical tests were carried out in 5 mM K3[Fe(CN)6]/K4[Fe(CN)6]/in 0.01 M PBS that was previously deaerated. The voltammetric behavior of the modified gold electrode in the presence of a simple redox system, K4[Fe(CN)6] in the supporting electrolyte of 0.1 M KCl, was compared with that of the bare electrode.
The validation of immunosensors included the optimization of time needed for protein N to be detected, defining the limit of the detection, and cross-reactivity test with pathogens other than the target pathogen. The plots show detection of target pathogen the immunosensor based on Au electrode were prepared according to the presented procedure then the solution of protein N with a concentration of 266 μg/mL (positive sample) was diluted in 0.1 PBS was spotted on the surface of each electrode and incubated at a temperature of 5 °C for different periods. The last step of sensors validation was the cross-reactivity tests were performed with different pathogens and patients' saliva swabs sick with COVID-19 as positive samples. To prepare saliva swabs for the electrochemical measurement 1 mL of it was diluted in the 1 mL of electrolyte utilized in the whole experiment, but with twice time higher concentration of electrochemical species to obtain the same concentration of 5mM [Fe(CN)6]3-/4- after dilution. The 30 μL of that mixture was then spotted on the surface of each electrode and left for the incubation time estimated in previous measurements for each electrode material. In cross-reactivity tests the same sample of saliva swab was used for multiple samples of prepared sensors, ensuring better reproducibility and comparability of results. As pre-diagnosed infected swabs contained Streptococcus pyogenes (S.py) (Ct = 41), Streptococcus pneumonia (S.pn) (Ct = 43), and Hemophilus influenzae (Hi) (Ct = 45).
These results are part of the manuscript published in Biosensors and Bioelectronics: DOI 10.1016/j.bios.2022.114222
Plik z danymi badawczymi
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} gdzie pojedyncza część pliku jest wielkości 512 MBPrzykładowy skrypt do wyliczenia:
https://github.com/antespi/s3md5
Informacje szczegółowe o pliku
- Licencja:
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otwiera się w nowej karcie
CC BYUznanie autorstwa - Dane surowe:
- Dane zawarte w datasecie nie zostały w żaden sposób przetworzone.
Informacje szczegółowe
- Rok publikacji:
- 2022
- Data zatwierdzenia:
- 2022-08-09
- Język danych badawczych:
- angielski
- Dyscypliny:
-
- nauki chemiczne (Dziedzina nauk ścisłych i przyrodniczych)
- DOI:
- Identyfikator DOI 10.34808/nq8v-4a13 otwiera się w nowej karcie
- Finansowanie:
- Weryfikacja:
- Politechnika Gdańska
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