Activity assay of O-Acetyl-L-homoserine sulfhydrylase (CaMet15p).

The study aimed to obtain conditions optimal for the activity of O-acetyl-L-homoserine sulfhydrylase (CaMet15p) and a method suitable for its measurement. The selection of appropriate reaction conditions included the identification of the reaction buffer and its pH, substrate and enzyme concentrations. The activity of CaMet15p was measured by the detection...

Effect of C-1748 derivatives on the biofilm formation of C. albicans ATCC 10231 cells

The datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.

Effect of C-1748 derivatives on the morphological transformation of C. albicans ATCC 10231 cells

The datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on morphological transformation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.

The viscometric evaluation of the gelation rate the chitosan-metal oxide hydrogel systems.

The dataset shows the change in the viscosity of a hydrogel medium over time. Viscometric measurement was used as a universal method for determining the cross-linking rate of chitosan hydrogel using metal oxides or other solutions with a negative net charge. The method was prepared as a method of predicting the kinetics of additive manufacturing using...

The effect of zinc oxide concentration on dry matter content of chitosan gels in carbonic acid.

The dataset contains results determining the effect of zinc oxide addition at different concentrations on the dry matter content of chitosan gels in carbonic acid after a specified time. Zinc oxide at concentrations of 0.5 and 1% was added as a powder to a 1% solution of chitosan in carbonic acid and then homogenized. Differences were observed in the...

Involvement of human glutathione S-transferase M1-1 in the glutathione conjugation of the antitumor unsymmetrical bisacridine derivative C-2028.

Unsymmetrical bisacridines (UAs) are a novel potent class of antitumor-active therapeutics. The aim of this study was to investigate the possible role of human glutathione S-transferase M1-1 (hGSTM1-1) in the glutathione (GSH) conjugation of a representative UA, C‑2028.

Determination the method of free alcohol fermented beverage production based on roasted raw materials.

Data set presents the results of monosaccharides and ethyl alcohol determination in samples of fermented cereal drink with increased health value based on barley malt and roasting of rye, chicory and beetroot, and with the addition of hops. The HPLC method with RID detector was used for determinations

Determination of changes in viscosity of high-fructose syrup stability of pectin hydrogels depending on the temperature (25-96 Celsius degrees).

The rheological characteristics of the high-fructose syrup modified stability of pectin (added in a  concentration 2% m/m) were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 25 spindle and a shear rate of 0,22 s-1 in the temperature range 25-96 ° C. The data allowed selection of optimal conditions for the...

Effect of Jerusalem artichoke addition on texture profile of bread

The dataset contains the results of Jerusalem artichoke addition on the texture of wheat bread. The following bread variants were tested: control bread, bread with 15% and 30% Jerusalem artichoke addition. Measurements were made immediately after baking. On the basis of data, the following parameters were determined: hardness, elasticity, cohesion and...

The effect of the flaxseed addition on the texture of wheat bread

The dataset contains the results of flaxseed addition on the texture of toasted bread. The following bread variants were tested: control bread, bread with 8% and 12% linseed addition and competitive bread. Measurements were made immediately after baking and after 4 days. On the basis of data the following parameters were determined: hardness, elasticity,...

The effect of flaxseed addition on the quality and basic composition of wheat bread

The dataset includes the effect of flaxseed addition on the properties of toasted bread bakery products such as basic composition, moisture content immediately after baking and after a defined time, porosity, acidity and total baking loss. The following bread variants were investigated: control bread, bread with 8% and 12% flaxseed addition and competitive...

The effect of wax foundation addition to PCL filaments on mechanical properties.

The dataset includes the effect of wax foundation addition on the basic mechanical properties of the filaments. PCL and wax foundation addition at 10 and 15% were used for extrusion. The mechanical properties of the resulting filaments were evaluated by a double compression test using an Instron model 5543 universal testing machine. Parameters such...

The effect of raw material type on endotoxin content .

The datasets contain the results of determining the effect of raw material type on endotoxin content as well as the effect of purification methods. The endotoxin concentration was determined using the PyroGene Recombinant Factor C Endpoint Fluorescent Assay. Beckmann-Kenko sodium alginate and Chemat sodium salt of alginic acid were used for the assays....

The effect of chitosan concentration and molecular weight on endotoxin content

The dataset includes results to determine the effect of chitosan concentration and molecular weight on endotoxin content as well as the effect of purification methods. The endotoxin concentration was determined using the PyroGene Recombinant Factor C Endpoint Fluorescent Assay. Chitosan 0.6% in 0.1M glycolic acid from Chemat LMW, MMW, and HMW were used...

Determination of the total number of microorganisms in a model food product preserved by CS-2a and CS-2b.

The dataset contains the results of determination of the total number of microorganisms in a model food product after the use of the innovative preservatives: CS-2a  and CS-2b and control (K) after seven days of refrigerated storage of the product. The test was performed using the surface inoculation method.

Test of the antimicrobial properties against Staphylococcus aureus of the innovative CS-2a preservative.

The dataset contains the results of a single series of determinations of the antimicrobial properties against Staphylococcus aureus of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on Baird-Parker Agar) at specified intervals (0 min.  and after 1 day-24 h of incubation ). This...

Test of the antimicrobial properties against E. coli of the innovative CS-2a preservative.

The dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on chromogenic Coliform Agar) at specified intervals (0 min. [cs2a 0] and after 1 day-24 h of incubation [cs2a...

Test of the antimicrobial properties against E. coli of the innovative CS-2b preservative.

The dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli of the innovative CS-2 b preservative in the solution of model fluids.During the test, the infected product is inoculated (on chromogenic Coliform Agar) at specified intervals (0 min. [cs2b 0] and after 1 day-24 h of incubation [cs2b...

Test of the antimicrobial properties against Listeria monocytogenes of the innovative CS-2a preservative.

The dataset contains the results of a single series of determinations of the antimicrobial properties against Listeria monocytogenes  of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on Oxford Agar) at specified intervals (0 min. [Listeria cs2a 0] and after 1 day-24 h of incubation...

Test of the antimicrobial properties against Listeria monocytogenes of the innovative CS-2b preservative.

The dataset contains the results of a single series of determinations of the antimicrobial properties against Listeria monocytogenes  of the innovative CS-2 b preservative in the solution of model fluids.During the test, the infected product is inoculated (on Oxford Agar) at specified intervals (0 min. [Listeria cs2b 0] and after 1 day-24 h of incubation...

The estrogenicity of wastewater samples.

The principle of operation of the kit is based on the fact that the tested sample with endocrine activity (containing compounds capable of binding to the human estrogen receptor α, hERα) releases the fluorescently labeled ligand (FluormoneTM ES2) from the receptor-ligand complex, which results in a reduction of the polarization value fluorescence. The...

ArchBGal32cB 441Glu mutein gene analysis dataset

Determination of changes in viscosity of pectin gel depending on shear rate (1.1 to 55 s-1).

The rheological characteristic of pectin gel (with concetration 0,45% m/m,  in three independent repetition of sample) was made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 25 spindle and shear rates from 1.1 to 55 s-1.The data allowed to characterize the used hydrogel preparations and assess their functional...

The effect of changes in the concentration of hydroxypropylcellulose on the rheological stability of pectin hydrogels.

The rheological characteristics of the pectin hydrogel modified by hydroxypropylcellulose (added in a  concetration range from 0.1 to 0.55% m/m) were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 25 spindle and a shear rate of 0,22 s-1 in the temperature range 19-96 ° C.The data allowed selection of optimal...

Determination of changes in viscosity of pectin gel depending on shear rate (1.7 to 85 s-1).

The rheological characteristic of pectin gel (with concetration 0,25% m/m,  in two independent repetitions) wasmade on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 27 spindle and shear rates from 1.7 to 85 s-1, in temperature of 25 C degee .The data allowed to characterize the used hydrogel preparations and assess...

Determination of sex hormone concentration in wastewater samples.

Concentration levels of five selected steroid hormones in influent and effluent waters from wastewater treatment plant were determined using SPE(C18)-HPLC-ESI/MS/MS method.  Sex hormones like: estradiol, estriol, estrone, 17-α-ethynyloestradiol, progesterone were present in all influent wastwater samples.

Influence of the presence of rhamnolipids and ionic cross-linking conditions on the mechanical properties of alginate hydrogels.

The dataset contains the results of determination the effect of rhamnolipids concentration, calcium chloride concentration and ionic cross-linking time on the mechanical properties of alginate hydrogels prepared by immersing the alginate mixture limited by the dialysis membrane in an appropriate cross-linking solution containing calcium ions. The mechanical...

Determination of changes in viscosity of poly(acrylic acid) PAA solutions depending on shear rate.

The rheological characteristics of poly(acrylic acid) PAA  solutions were based on viscosity measurements using a Brookfield viscometer, using LV SC4 - 27 spindles and shear rates from 1,7 to 39 s-1 (Fig. 1). PAA solutions with were prepared in water. The data allowed to characterize the used PAA and assess their functional parameters as rheology modifying...

Determination of changes in viscosity of refined oil depending on the temperature (19-96 Celsius degrees).

The rheological characteristics of the refined oil were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 18 spindle and a shear rate of 52.8 s-1 in from temperature 19-96 °C.

Determination of changes in viscosity of hydrogel depending on shear rate (6.6 to 330 s-1).

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 18 spindle and shear rates from 6.6 to 330 s-1.

Determination of changes in viscosity of hydrogel depending on shear rate (1.7 to 34 s-1).

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 27 spindle and shear rates from 1.7 to 34 s-1.

Determination of changes in viscosity of hydrogel depending on shear rate (1.1 to 55 s-1).

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 25 spindle and shear rates from 1.1 to 55 s-1.

The profiles and spectrum of aqua solution of tannic acid by HPLC-DAD-MS analysis.

The profiling of tannic acid is determined by examining the content and proportion / ratio of gallotannins [Aras et al. 2016, Clifford et al. 2007, Gauri et al. 2012, Nishizawa et al. 1982]. In order to check the ion mass distribution (TIC) in an aqueous solution of commercial tannic acid, a direct sample injection (TA) was performed into the mass spectrometer....

The assessment of microbiological antimicrobial properties of PE film loaded with nanozinc filler

The dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli and S. aureus of polyethylene films containing the nanozinc filler.

Production of un-matured white soft cheese.

The video presents instructional work on the production of un-matured white soft cheese. 

The influence of the temperature treatment method on the quality of French fries.

The video presents instructional work on the temperature treatment of French fries and the assessment of oil changes during frying. The results of the measurements show that placing the fries in cold oil, heating them to the frying temperature compared to the conventional method of frying, reduces the amount of primary oxidation products and free fatty...

Determination of the content of saccharides and ethanol in samples of fermented beverages

The data set presents the results of measurements of the content of mono- and disaccharides: glucose, maltose, fructose and ethanol in samples of beverages fermented by high performance liquid chromatography (HPLC-RID).

The PolarScreen™ Estrogen Receptor Competitor Assays for determination of estradiol equivalent concentrations (EEQs) in sewage and drinking water samples.

Municipal waste waters are one of the main sources of estrogenic compounds in aquatic environments. Feminization of fish downstream of Waste Water Treatment Plants (WWTPs) discharges has been observed worldwide. Some estrogenic chemicals, particularly steroid estrogens, are known to cause disruption of the endocrine system of fishes and abnormalities...