A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry - Publikacja - MOST Wiedzy

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A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry

Abstrakt

Ibuprofen is widely used in human and veterinary medicine for the treatment of chronic pain as well as rheumatic and musculoskeletal disorders. However, t he anal- gesic and anti-inflammatory properties of Ibuprofen have contributed to frequent drug abuse in equestr ian s ports. A sensitive and rapid gas chromatography with tandem mass spectrometry based method with a simple liquid-liquid extraction and deriva- tization requiring 200 μL volume of sample and 2 mL of extraction solvent for the simultaneous determination of ibuprofen and its metabolites was developed. The pro- posed procedure was optimized and validated according to the principles for bioana- lytical methods. The assay achieved satisfactory validation parameters, namely, recov- ery (92.2–105%), interday accuracy (92.5–106%), and precision (0.3–4.4%) for all investigated compounds as well as limits of quantification of 50 ng/mL for ibuprofen, 2-hydroxyibuprofen, and carboxyibuprofen, 25 ng/mL for 1-hydroxyibuprofen and 100 ng/mL for 3-hydroxyibuprofen. The applicability of the method was evaluated by the analysis of five real urine samples collected from different horses after drug administration. In view of the low limits of quantification, high selectivity, repeata- bility, and recovery, t he procedure can be utilized for laboratory applications, includ- ing the control of ibuprofen abuse in equestrian sports for anti-doping purposes and drug/pharmaceutical mentality investigations.

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Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
JOURNAL OF SEPARATION SCIENCE nr 41, strony 3881 - 3891,
ISSN: 1615-9306
Język:
angielski
Rok wydania:
2018
Opis bibliograficzny:
Waraksa E., Woźniak M. K., Kłodzińska E., Wrzesień R., Bobrowska-Korczak B., Namieśnik J.: A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry// JOURNAL OF SEPARATION SCIENCE. -Vol. 41, iss. 20 (2018), s.3881-3891
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1002/jssc.201800614
Weryfikacja:
Politechnika Gdańska

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