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Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications
Abstrakt
This paper focuses on the development of a novel electrode based on boron-doped diamond nanosheet full-volume-enriched screen-printed carbon electrodes (BDDPE) for use as an impedimetric biosensor. Impedimetric biosensors offer high sensitivity and selectivity for virus detection, but their use as point-of-care devices is limited by the complexity of nanomaterials’ architecture and the receptor immobilisation procedures. The study presents a two-step modification process involving the electroreduction of diazonium salt at the BDDPE and the immobilisation of antibodies using zero-length cross-linkers for a selective impedimetric biosensor of Haemophilus influenzae (Hi). The incorporation of diamond nanosheets into BDDPE leads to enhanced charge transfer and electrochemical behaviour, demonstrating greatly improved electrochemically active surface area compared with unmodified screen-printed electrodes (by 44% and 10% on average for [Ru(NH3)6]Cl2 and K3[Fe(CN)6], respectively). The presented sensing system shows high specificity towards protein D in Hi bacteria, as confirmed by negative controls against potential interference from other pathogens, with an estimated tolerance limit for interference under 12%. The Hi limit of detection by electrochemical impedance spectroscopy was 1 CFU/mL (measured at − 0.13 V vs BDDPE pseudo-reference), which was achieved in under 10 min, including 5 min sample incubation in the presence of the analyte.
Cytowania
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Autorzy (7)
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Mateusz Brodowski
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Sawczak Mirosław dr hab. inż.
- Instytut Maszyn Przepływowych PAN
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Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s00604-023-05991-w
- Licencja
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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MICROCHIMICA ACTA
ISSN: 0026-3672 - Język:
- angielski
- Rok wydania:
- 2023
- Opis bibliograficzny:
- Ficek M., Cieślik M., Janik M., Brodowski M., Mirosław S., Bogdanowicz R., Ryl J.: Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications// MICROCHIMICA ACTA -,iss. 190 (2023), s.410-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s00604-023-05991-w
- Źródła finansowania:
- Weryfikacja:
- Politechnika Gdańska
Powiązane datasety
- dane badawcze Topography studies of screen-printed carbon electrodes (SPCE) with boron-doped diamond foils
- dane badawcze Electrochemical studies for screen-printed carbon electrodes (SPCE) with boron-doped diamond foils
- dane badawcze Chemical and structural studies of screen-printed carbon electrodes (SPCE) with boron-doped diamond foils
- dane badawcze Performance of Haemophilus influenzae impedimetric biosensors based on screen-printed carbon electrodes (SPCE) with boron-doped diamond foils
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