Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )

Joanna Cieślewicz; Marcin M. Kamiński; Marian Antoni Kamiński

doi:10.1002/jssc.201800506

Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )

Abstrakt

The review presents general principles for choosing optimal conditions for ecdysteroid separation, identification, and isolation using HPLC/TLC techniques in RP, NP- HILIC or NP modes. Analytics of ecdyteroids pose a still insufficiently resolved problem. Plant-derived ecdysteroids are a point of interest of pharmaceutical industry and sport medicine due to their postulated adaptogenic and anabolic properties. In insects, ecdysteroids regulate larval transformation. Maral root (Rhaponticum carthamoides, Leuzea carthamoides), traditional Siberian folk-medicine plant used as stimulant to boost overall health and fitness, is a particularly rich source of a wide variety of phytoecdysteroids. The similarity of molecular structures of ecdysteroids present in its extracts together with high content of unrelated compounds of similar chromatographic characteristics makes optimization of separation, identification and isolation of ecdysteroids a difficult analytical task. In that respect, two-dimensional separations, two-dimensional separations, 2D HPLC or 2D TLC, could be of use. For identification, the hyphenated techniques are particularly important. Thus, comprehensive overview of MS spectral parameters of ecdysteroids is provided. Described principles could easily be applied for separation of ecdysteroids in extracts from other sources. They are also useful for development of separation procedures for isolation of ecysteroids in preparative-scale applications.

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Autorzy (3)

Joanna Cieślewicz (dawniej: Joanna Głazowska)
Marcin M. Kamiński
Marian Antoni Kamiński prof. dr hab. inż.

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Słowa kluczowe

LEUZEA CARTHAMOIDES , LIQUID CHROMATOGRAPHY OF PLANT SECONDARY METABOLITES, MARAL ROOT, PHYTOECDYSTEROIDS, RHAPONTICUM CARTHAMOIDES

Informacje szczegółowe

Kategoria:: Publikacja w czasopiśmie
Typ:: artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:: JOURNAL OF SEPARATION SCIENCE nr 41, strony 4304 - 4314,
ISSN: 1615-9306
Język:: angielski
Rok wydania:: 2018
Opis bibliograficzny:: Głazowska J., Kamiński M. M., Kamiński M. A.: Chromatographic separation, determination and identification of ecdysteroids: Focus on Maral root (Rhaponticum carthamoides, Leuzea carthamoides )// JOURNAL OF SEPARATION SCIENCE. -Vol. 41, iss. 23 (2018), s.4304-4314
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1002/jssc.201800506
Weryfikacja:: Politechnika Gdańska