DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation - Publikacja - MOST Wiedzy

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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation

Abstrakt

A new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific linker. Only DNA fragmens which consist pehX gene are amplified from the ligation product by PCR. Variety of PCR product length can differentiate the K. oxytoca strains among species.

Informacje szczegółowe

Kategoria:
Inne
Typ:
suplement, wydanie specjalne, dodatek
Opublikowano w:
Acta Biochimica Polonica nr 58,
ISSN: 0001-527X
Tytuł wydania:
Acta Biochimica Polonica.
Język:
angielski
Rok wydania:
2011
Opis bibliograficzny:
Stojowska K., Krawczyk B., Kur J.: DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation// Acta Biochimica Polonica. -Vol. 58., nr. suppl. 4 (2011),
Weryfikacja:
Politechnika Gdańska

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