Wyszukiwarka
Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections
Abstrakt
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are among the most common bacterial infectious diseases in the developed world. The urovirulence of UPEC is mainly associated with the surface-exposed fimbrial adhesins and adhesins of the autotransporter (AT) family. The best studied of theses proteins is antigen 43 (Ag43) mediating cell aggregation, adhesion and biofilm development as the causes of chronic UTIs. E. coli IH11128 Dr+ (dra+) strain of the Dr/Afa+ family of adhesins, possesses two major surface-exposed virulence factors (VFs), Dr fimbrial polyadhesin and DraD protein (fimbrial tip subunit or protein component of adhesive sheath). Here, we identified for the first time the agn43 gene encoding Ag43 antigen in the wild-type clinical isolate of UPEC Dr+ as a new VF not tested yet. We also found that the Dr fimbrial expression which like Ag43 is under the control of phase-variable mechanism did not exclude Ag43 surface presentation. However, the presence of Dr fimbriae supported by other structures on the cell surface caused a physical neutralization of Ag43-mediated autoaggregation during in vitro growth. The fimbrial bundling further increased the distance between the adjacent Ag43+ cells thus preventing head-to-tail association between surface-exposed Ag43 subunits and their interactions with the host cells. The investigations showed that Ag43 did not act as a specific adhesin and invasin conversely to the major VFs of E. coli Dr+ but play significant role in the viability and metabolic activity of bacterial cells forming biofilm and survival of bacteria within invaded epithelial cells.
Cytowania
-
1 0
CrossRef
-
0
Web of Science
-
1 2
Scopus
Autorzy (4)
Cytuj jako
Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1099/mic.0.000072
- Licencja
- Copyright (2015 Microbiology Society)
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuł w czasopiśmie wyróżnionym w JCR
- Opublikowano w:
-
MICROBIOLOGY-SGM
nr 161,
strony 1034 - 1049,
ISSN: 1350-0872 - Język:
- angielski
- Rok wydania:
- 2015
- Opis bibliograficzny:
- Zalewska-Piątek B., Piątek R., Olszewski M., Kur J.: Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections// MICROBIOLOGY-SGM. -Vol. 161, (2015), s.1034-1049
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1099/mic.0.000072
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 192 razy
Publikacje, które mogą cię zainteresować
A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin
- B. Zalewska-Piątek,
- M. Olszewski,
- T. Lipniacki
- + 7 autorów