Identification of novel psychoactive substances 25B-NBOMe and 4-CMC in biological material using HPLC-Q-TOF-MS and their quantification in blood using UPLC–MS/MS in case of severe intoxications
This paper describes cases of poisoning caused by new psychoactive substances such as: 25BNBOMe (2-(4-bromo-2,5-dimethoxyphenyl)-N-[(2-methoxyphenyl)methyl]ethanamine) and 4-CMC (1-(4-chlorophenyl)-2-(methylamino)-1-propanone). The analytical procedure includes rapid and selective method for the extraction and determination of 4-CMC and 25B-NBOMe in blood samples using UPLC–MS/MS technique. To the best of our knowledge,this is thefirst report,thatinvolves a fully validated method for quantification of new-designer drug – 4-CMC in postmortem blood samples. The biological material was also analyzed with the use of routine analytical methods: immunochemical techniques, gas chromatography with flame ionization detection and gas chromatography with electron impact mass spectrometry. The results of real samples analyses correspond to possible toxicological effects: death resulting from 25B-NBOMe - mediated hallucinations (661 ng/mL of 25B-NBOMe and 0.887 ng/mL of 4- CMC), fatal overdose of 25B-NBOMe and 4-CMC (66.5 ng/mL of 25B-NBOMe and 2.14 ng/mL of 4-CMC) and non-fatal intoxication of these drugs (38.4 ng/mL of 25B NBOMe and 0.181 ng/mL of 4-CMC). Additionally, O-demethylathed O, O-bis-demethylathed and glucuronidated metabolites of 25B-NBOMe in biological specimens were detected.
Marek Wiergowski, Justyna Aszyk, Michał Kaliszan, Kamila Wilczewska, Jacek Sein Anand, Agata Kot-Wasik, Zbigniew Jankowski. (2017). Identification of novel psychoactive substances 25B-NBOMe and 4-CMC in biological material using HPLC-Q-TOF-MS and their quantification in blood using UPLC–MS/MS in case of severe intoxications. Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences, 1041-1042, 1-10. https://doi.org/10.1016/j.jchromb.2016.12.018
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