Isolation and some properties of collagen from the backbone of Baltic cod(Gadus morhua) - Publikacja - MOST Wiedzy

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Isolation and some properties of collagen from the backbone of Baltic cod(Gadus morhua)

Abstrakt

Ossein from Baltic cod backbone was obtained after extraction of non-collagenous protein with 0.1 M NaOH solution and demineralization with 1.0 M HCl solution. The extractions were performed at 4 C for24, 48 and 72 h using a solid/solution ratio from 1:4 to 1:8 (w/v). After 48 h of extraction in 0.5 M acetic acid only about 25% of collagen was dissolved. After 48 h of extraction at optimal concentration of pepsin(4 mg/g ossein) the collagen was dissolved almost completely. Collagen solubilized in acetic acid could bealmost completely precipitated with k-carrageenan, at protein/polysaccharide ratio 1:1 (w:w). The denaturation temperature of Baltic cod backbone collagen was 14.4 C and the conversion factor of hydroxyproline to collagen was 15.7.

Elżbieta Żelechowska, Maria Sadowska, Magdalena Turk. (2010). Isolation and some properties of collagen from the backbone of Baltic cod(Gadus morhua), 24(iss. 4), 325-329.

Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
FOOD HYDROCOLLOIDS nr 24, strony 325 - 329,
ISSN: 0268-005X
Język:
angielski
Rok wydania:
2010
Opis bibliograficzny:
Żelechowska E., Sadowska M., Turk M.: Isolation and some properties of collagen from the backbone of Baltic cod(Gadus morhua)// FOOD HYDROCOLLOIDS. -Vol. 24, nr. iss. 4 (2010), s.325-329

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