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Mangiferin Affects Melanin Synthesis by an Influence on Tyrosinase: Inhibition, Mechanism of Action and Molecular Docking Studies
Abstrakt
Mangiferin is a strong antioxidant that presents a wide range of biological activities. The aim of this study was to evaluate, for the first time, the influence of mangiferin on tyrosinase, an enzyme responsible for melanin synthesis and the unwanted browning process of food. The research included both the kinetics and molecular interactions between tyrosinase and mangiferin. The research proved that mangiferin inhibits tyrosinase activity in a dose-dependent manner with IC50 290 +/− 6.04 µM, which was found comparable with the standard kojic acid (IC50 217.45 +/− 2.54 µM). The mechanism of inhibition was described as mixed inhibition. The interaction between tyrosinase enzyme and mangiferin was confirmed with capillary electrophoresis (CE). The analysis indicated the formation of two main, and four less significant complexes. These results have also been supported by the molecular docking studies. It was indicated that mangiferin binds to tyrosinase, similarly to L-DOPA molecule, both in the active center and peripheral site. As it was presented in molecular docking studies, mangiferin and L-DOPA molecules can interact in a similar way with surrounding amino acid residues of tyrosinase. Additionally, hydroxyl groups of mangiferin may interact with amino acids on the tyrosinase external surface causing non-specific interaction.
Cytowania
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5
CrossRef
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0
Web of Science
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6
Scopus
Autorzy (8)
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Justyna Stefanowicz-Hajduk
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Szymon Dziomba
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Rafal Halasa
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Renata J. Ochocka
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Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3390/antiox12051016
- Licencja
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otwiera się w nowej karcie
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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Antioxidants
nr 12,
ISSN: 2076-3921 - Język:
- angielski
- Rok wydania:
- 2023
- Opis bibliograficzny:
- Hering A., Stefanowicz-Hajduk J., Dziomba S., Halasa R., Krzemieniecki R., Sappati S., Baginski M., Ochocka R. J.: Mangiferin Affects Melanin Synthesis by an Influence on Tyrosinase: Inhibition, Mechanism of Action and Molecular Docking Studies// Antioxidants -Vol. 12,iss. 5 (2023), s.1016-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3390/antiox12051016
- Źródła finansowania:
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- COST_FREE
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 48 razy
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