MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products

Katarzyna Nalazek-Rudnicka; Ilona Edyta Kłosowska-Chomiczewska; Andrzej Wasik; Adam Macierzanka

doi:10.1016/j.foodchem.2019.01.007

MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products

Abstrakt

The abundance of protein markers in different types of meat cuts was explored in the context of authentication of raw meat (pork, beef and chicken) and processed meat products. Peptides originating from myoglobin (Mb) and myosin (My) were analyzed using multiple reaction monitoring mass spectrometry (MRM–MS). Analytical protocol was optimized for good repeatability (CV < 10%) and high sensitivity. The MS signal intensity of Mb marker peptides in raw pork depended significantly on the cut type (e.g. ham vs knuckle). Importantly, a similar pattern in the abundance of the marker peptides was found for processed meat products made of different types of pork cuts, despite the food processing applied. This suggests the protocol can be used for authentication of raw pork cuts and processed products made of different cuts of pork. More uniform contents of Mb markers were found in raw beef cuts, and for My markers in raw chicken cuts.

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Autorzy (4)

Katarzyna Nalazek-Rudnicka
Ilona Edyta Kłosowska-Chomiczewska dr inż.
Andrzej Wasik prof. dr hab. inż.
Adam Macierzanka dr hab. inż.

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Wersja publikacji: Accepted albo Published Version
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Informacje szczegółowe

Kategoria:: Publikacja w czasopiśmie
Typ:: artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:: FOOD CHEMISTRY nr 283, strony 367 - 374,
ISSN: 0308-8146
Język:: angielski
Rok wydania:: 2019
Opis bibliograficzny:: Nalazek-Rudnicka K., Kłosowska-Chomiczewska I., Wasik A., Macierzanka A.: MRM–MS of marker peptides and their abundance as a tool for authentication of meat species and meat cuts in single-cut meat products// FOOD CHEMISTRY. -Vol. 283, (2019), s.367-374
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.foodchem.2019.01.007
Weryfikacja:: Politechnika Gdańska