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Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation
Abstrakt
The 21st century has already brought us a plethora of new threats related to viruses that emerge in humans after zoonotic transmission or drastically change their geographic distribution or prevalence. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first spotted at the end of 2019 to rapidly spread in southwest Asia and later cause a global pandemic, which paralyzes the world since then. We have designed novel immunosensors targeting conserved protein sequences of the N protein of SARS-CoV-2 based on lab-produced and purified anti-SARS-CoV-2 nucleocapsid antibodies that are densely grafted onto various surfaces (diamond/gold/glassy carbon). Titration of antibodies shows very strong reactions up to 1:72 900 dilution. Next, we showed the mechanism of interactions of our immunoassay with nucleocapsid N protein revealing molecular recognition by impedimetric measurements supported by hybrid modeling results with both density functional theory and molecular dynamics methods. Biosensors allowed for a fast (in less than 10 min) detection of SARS-CoV-2 virus with a limit of detection from 0.227 ng/ml through 0.334 ng/ml to 0.362 ng/ml for glassy carbon, boron-doped diamond, and gold surfaces, respectively. For all tested surfaces, we obtained a wide linear range of concentrations from 4.4 ng/ml to 4.4 pg/ml. Furthermore, our sensor leads to a highly specific response to SARS-CoV-2 clinical samples versus other upper respiratory tract viruses such as influenza, respiratory syncytial virus, or Epstein-Barr virus. All clinical samples were tested simultaneously on biosensors and real-time polymerase chain reactions.
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Autorzy (25)
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Wioleta Białobrzeska dr
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Silvio Osella
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Andres Jaramillo-Botero
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Yanina Dashkevich
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Tomasz Łęga
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Natalia Malinowska
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Zofia Cebula
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Daniel Firganek
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Ewelina Bięga mgr
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Karolina Dziąbowska
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Marcin Kowalski
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Mirosława Panasiuk
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Beata Gromadzka
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Sabina Żołędowska dr
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Dawid Nidzworski dr inż.
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Krzysztof Pyrć
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William A. Goddard III prof
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Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.bios.2022.114222
- Licencja
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otwiera się w nowej karcie
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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BIOSENSORS & BIOELECTRONICS
nr 209,
ISSN: 0956-5663 - Język:
- angielski
- Rok wydania:
- 2022
- Opis bibliograficzny:
- Białobrzeska W., Ficek M., Dec B., Osella S., Trzaskowski B., Jaramillo-Botero A., Pierpaoli M., Rycewicz M., Dashkevich Y., Łęga T., Malinowska N., Cebula Z., Bigus D., Firganek D., Bięga E., Dziąbowska K., Brodowski M., Kowalski M., Panasiuk M., Gromadzka B., Żołędowska S., Nidzworski D., Pyrć K., Goddard Iii W. A., Bogdanowicz R.: Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation// BIOSENSORS & BIOELECTRONICS -Vol. 209, (2022), s.114222-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.bios.2022.114222
- Źródła finansowania:
- Weryfikacja:
- Politechnika Gdańska
Powiązane datasety
- dane badawcze The electrochemical response to glassy carbon electrode modification steps towards viral electrochemical immunosensor preparation
- dane badawcze Scanning electron microscopy (SEM) images of the boron-doped carbon nanowalls surfaces
- dane badawcze The electrochemical response to Au electrode modification steps towards viral electrochemical immunosensor preparation
- dane badawcze Scanning electron microscopy (SEM) images of the boron-dopied diamond surfaces
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