Wyszukiwarka
Quantification of unconjugated and total ibuprofen and its metabolites in equine urine samples by gas chromatography–tandem mass spectrometry: Application to the excretion study
Abstrakt
Ibuprofen (IBU) is a widely used nonsteroidal anti-inflammatory drug (NSAID) in human and also veterinary medicine. NSAIDs are usually highly metabolized compounds in horses; they are often present in equine urine, mostly in their conjugated forms (glucuronides). Thus, hydrolysis to cleave the glucuronide linkage prior to anti-doping analysis is often necessary for improving detection. In this study, unconjugated and total IBU and its metabolites were determined by GC–MS/MS in equine urine samples collected after drug administration to six horses (mares and geldings). The results indicated that enzymatic hydrolysis using β-glucuronidase from E. coli improved the detection of IBU and its phase I metabolites in equine urine samples; in a few cases, this method allowed to monitor analytes for a longer period of time. The percentages of conjugated analytes were within 5.4–48.4% for IBU and in the range of 0.2–55.5% for phase I metabolites of IBU. Unchanged IBU was found at the highest concentration levels in equine urine samples collected after drug administration; and also parent compound lasted for a longer period of time in equine urine than its phase I metabolites, with the exception of samples collected from one horse, in which 2-OH IBU was detectable for a longer duration than IBU.
Cytowania
-
4
CrossRef
-
0
Web of Science
-
5
Scopus
Autorzy (8)
-
Ewa Kłodzińska dr. hab.
-
Mariusz Ozimek
-
Robert Wrzesień
-
Barbara Bobrowska-Korczak
-
Jacek Namieśnik prof. dr hab. inż.
Cytuj jako
Pełna treść
pełna treść publikacji nie jest dostępna w portalu
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
-
MICROCHEMICAL JOURNAL
nr 150,
ISSN: 0026-265X - Język:
- angielski
- Rok wydania:
- 2019
- Opis bibliograficzny:
- Waraksa E., Woźniak M., Banaszkiewicz L., Kłodzińska E., Ozimek M., Wrzesień R., Bobrowska-Korczak B., Namieśnik J.: Quantification of unconjugated and total ibuprofen and its metabolites in equine urine samples by gas chromatography–tandem mass spectrometry: Application to the excretion study// MICROCHEMICAL JOURNAL -Vol. 150, (2019), s.104129-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.microc.2019.104129
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 132 razy
Publikacje, które mogą cię zainteresować
Simultaneous determination of ibuprofen and its metabolites in complex equine urine matrices by GC-EI-MS in excretion study in view of doping control
- E. Waraksa,
- M. Wójtowicz-zawadka,
- D. Kwiatkowska
- + 4 autorów
Elimination of excretion of ibuprofen and its metabolites in horses
- E. Waraksa,
- M. Wójtowicz,
- D. Kwiatkowska
- + 1 autorów