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Abstrakt
The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist in intestinal lipolysis. However, human bile can potentially influence proteins that are largely resistant to gastric digestion, and which are mainly hydrolysed after they have been transferred to the small intestine. We used purified and food-grade bovine milk β-lactoglobulin (βLg) to assess the impact of bile salts (BS) on the in vitro gastrointestinal digestion of this protein. Quantitative analysis showed that the proteolysis rate increased significantly with increasing BS concentration. The effect was consistent regardless of whether individual BS or real human bile samples, varying in BS concentrations, were used. The total BS content of bile was more important than its BS composition in facilitating the proteolysis of βlg. We also show that the impact of human bile observed during the digestion of purified βLg and βLg-rich whey protein isolate can be closely replicated by the use of individual BS mixed with phosphatidylcholine. This could validate simple BS/phosphatidylcholine mixtures as human-relevant substitutes of difficult-to-obtain human bile for in vitro proteolysis studies.
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Autorzy (8)
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Robert Staroń
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Lukasz Krupa
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Neil Rigby
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Alan Mackie
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Krzysztof Gutkowski
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- Wersja publikacji
- Accepted albo Published Version
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Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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FOOD RESEARCH INTERNATIONAL
nr 145,
ISSN: 0963-9969 - Język:
- angielski
- Rok wydania:
- 2021
- Opis bibliograficzny:
- Dulko D., Staroń R., Krupa L., Rigby N., Mackie A., Gutkowski K., Wasik A., Macierzanka A.: The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin// FOOD RESEARCH INTERNATIONAL -Vol. 145, (2021), s.110413-
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.foodres.2021.110413
- Źródła finansowania:
-
- Działalność statutowa/subwencja
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 134 razy
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