Wyniki wyszukiwania dla: aflp - MOST Wiedzy

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Wyniki wyszukiwania dla: aflp

Wyniki wyszukiwania dla: aflp

  • Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples

    Publikacja
    • A. Białkowska
    • K. Szulczewska
    • J. Krysiak
    • T. Florczak
    • E. Gromek
    • H. Kassassir
    • J. Kur
    • M. Turkiewicz

    - POLAR BIOLOGY - 2017

    The Polish Arctowski Station is situated in the maritime Antarctic on the western shore ot' Admiralty Bay and encompasses terrestrial habitats which are not perma-nently covered by ice, in contrast to morę than 90% of the island's surface area. Over the past several decades, stud-ies exploring the soils of those habitats have revealed a considerable diversity of bacteria, filamentous fungi, and, to a lesser extent, yeasts; however,...

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  • ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping

    A DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....

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  • Diagnostyka molekularna w zakażeniach szpitalnych

    Publikacja

    Opisano najczęściej wykorzystywane techniki genotypowania w badaniach zakażeń szpitalnych z uwzględnieniem ich powtarzalności i potencjału różnicującego oraz problemów technicznych i ponoszonych kosztów. Makrorestrykcyjna analiza genomowego DNA z wykorzystaniem PFGE (ang. Pulsed Field Gel Electrophoresis)jest uważana za ''złoty standard'' w typowaniu molekularnym. Jednak jest ona czasochłonna, pracochłonna i dlatego nie nadaje...

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  • Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms

    Publikacja

    - Acta Biochimica Polonica - 2016

    A significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...

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  • The New LM-PCR/Shifter Method for the Genotyping of Microorganism

    Techniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...

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  • Ligation Mediated PCR methods, their possibilities and limitations

    Publikacja

    The application of molecular diagnostics for bacterial strain typing or identifying bacteria at the strain level is of particular importance at present. However, among the many genotyping methods that are currently available, no single one is universally ideal. In this article, we thus review the Ligation Mediated PCR group methods as prospects from which to choose. We discuss different strategies for selecting the amplified PCR...