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Katedra Technologii Leków i Biochemii
Dane badawcze
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wszystkich: 76
Katalog Danych Badawczych
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The analysis of the expression of TEL-F and IFNB1 genes in anthraquinone-treated NSCLC cells
Dane BadawczeThe data include Ct values of TEL-F and IFNB1 genes from samples subjected to long-term treatment with C4.
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Assay of Cystathionine-γ-synthase (CaStr2p) activity determination.
Dane BadawczeThe study aimed to obtain conditions optimal for the activity of Candida albicans cystathionine-γ-synthase (CaStr2p) determination. The selection of appropriate reaction conditions included the identification of the reaction buffer and its pH, substrate, and enzyme concentrations. The activity of Str2p was measured by the detection of decrease of the...
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Effect of antitumor compounds on the yeast topoisomerase II relaxation activity
Dane BadawczeThe datasets contain results of antitumor compounds* (known inhibitors of human topoisomerase alpha II) inhibitory activity against yeast topoisomerase II. DNA topoisomerases (Topo) are enzymes that catalyze changes in the spatial structure of DNA and play an important role in replication, transcription and recombination. Beyond their normal functions,...
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Overproduction of CaStr2p native and His-tag versions.
Dane BadawczeEnzyme of fungal L-methionine biosynthetic pathway: cystathionine-γ-synthase (CaStr2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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Investigation of the C-1311 glucuronidation: an electrochemical approach
Dane BadawczeThis study was undertaken to investigate the glucuronidation of the compound C-1311 (5-diethylaminoethylamino-8-hydroxyimidazoacridinone – the model anticancer acridine derivative) using electrochemistry/mass spectrometry (EC/MS) as a complementary technique to in vitro (liver microsomes) and in silico approaches.
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Sequencing results and other data presented in article published in Applied Microbiology and Biotechnology
Dane BadawczeThis folder contains sequences of the strains SzA14, SzA16 and SzB3. Other data presented in the article published in Applied Microbiology and Biotechnology have been also included.
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Probiotic strains from bee products - sequences and other data
Dane BadawczeThis folder contains seqences and other data necessary for evaluation of probiotic potential of bacteria islated from bee products.
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Antifungal activity of propolis extracts, screening and kinetic data
Dane BadawczeThis folder contains results of our studies aiming in determination of antifungal potential of ethanolic extracts of propolis and components of this product. In details, MIC, MFC values were determined and kill time assays were performed.
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Probiotic properties of bacterial strains isolated form bee products.
Dane BadawczeThis folder contains information about probiotic properties of bacterial strains isolated from bee products. Additionally information about antibiotic resistance are included.
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NADES propolis extracts - update 2023 and synthesis of derivatives of propolis ingredients
Dane BadawczeThis dataset contains results of our investigation aiming in determination of antimicrobial potential of the propolis extracts produced with deep eutectic solvents
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The analysis of the expression of hTERT in anthraquinone-treated NSCLC cells
Dane BadawczeThe datasets showcase an analysis of hTERT relative gene expression in NSCLC cells treated with anthraquinone, quantified as fold change compared to DMSO-treated control cells, using the comparative CT method.
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The effect of anthraquinone derivatives (C1-C9) on telomerase activity
Dane BadawczeThe data set contains images of the PCR products obtained from the Telomerase Repeated Amplification Protocol (TRAP). The effect of anthraquinone derivatives (C-C9) on telomerase activity was analyzed using lysates from drug-treatedcancer cell line.
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Antiviral activity of bee bread derived from polish apiaries.
Dane BadawczeBee bread is a product of fermentation of bee-collected pollen and revealed a high nutritional value. Other bee products, such as honey and propolis, are known for their antiviral activity, but bee bread is still under investigation, thus its antiviral potential is still unspecified. For investigation antiviral activity of bee bread samples, cytotoxicity...
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Analysis of cellular senescence following anthraquinone treatment in A549, H226, and H460 cells
Dane BadawczeThe datasets comprise microscopic images of A549, H226, and H460 cells following treatment with anthraquinones and staining with senescence-associated β-galactosidase. The images were captured using an Olympus BX60 microscope (Tokyo, Japan).
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Analysis of cell cycle distribution
Dane BadawczeThe dataset comprehensively details the distribution of A549, H226, and H460 cell lines in various cell cycle phases following treatment with the IC90 concentration of selected anthraquinones. The analysis was performed using the Flowing Software 2.
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Determination of the MIC (minimum inhibitory concentration) of new bisacridines IKE16-19, IKE21 and IE10 against C. glabrata clinical strains
Dane BadawczeThe datasets contain the results of determining the MIC value (minimum inhibitory concentration) of new bisacridines IKE16-19, IKE21 and IE10 against Candida glabrata clinical strains CZD 310, 373, 377, 513 and collection strain DSM 11226 by the modified M27-A3 specified by the CLSI.
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Effect of novel bisacridines (IKE16, IKE18, IKE19, IKE21) and IE10 on the biofilm formation of C. albicans ATCC 10231 cells
Dane BadawczeThe datasets contain the results of the impact of novel bisacridines (IKE16, IKE18, IKE19, IKE21) and IE10 on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.
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The absorption and fluorescence spectra of novel bisacridines (IKE15-19, IKE21) and IE10, potential antifungal agents
Dane BadawczeOptical measurements of novel bisacridines (IKE15-19, IKE21) and IE10 were conducted. The absorption spectra were recorded from 300 to 800 nm. The fluorescence emission spectra were determined with excitation and emission wavelengths described in the file. All measurements were recorded using a multiplate reader, Tecan Spark 10M.
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Initial geometries and MD trajectories of right- and left-handed G-quadruplexes
Dane BadawczeDataset contains initial geometries of 12 monomeric, 2-tetrad G-quadruplexes featuring three different loop lengths (T1, T2 and T3), adapted by the sequence GGTnGGTnGGTnGG where n=(1-3). For each loop length variant two directions of strand progression (clockwise (+) and anti-clockwise (-)) and two helicities (left-handed (LH) and right-handed (RH))...
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Wound healing migration assay
Dane BadawczeThe dataset presents the quantified percentage of wound closure, measured using ImageJ software. The progression of wound width over time was assessed following the treatment of A549, H226, and H460 cells with selected anthraquinones.
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Killing kinetics analysis of C-1311 derivative with octaarginine (Compound 1-R8) against Candida albicans
Dane BadawczeThe datasets contain the results of killing kinetics analysis of imidazoacridinone derivative and fluconazole against C. albicans ATCC 10231. The suspensions of Candida albicans ATCC 10231 cells (500 µL) in RPMI 1640 at cell density of 104 cells /mL were added to 500 µL of RPMI 1640 medium with various concentrations of the compounds, corresponding...
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Determination of the minimum inhibitory concentration of C-1330 derivatives (IKE9-IKE14) against Candida strains
Dane BadawczeThe datasets contain the results of determining the minimum inhibitory concentration of C-1330 derivatives (IKE9-IKE14) against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 by the modified M27-A3 specified by the CLSI.
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Effect of C-1748 derivatives on the biofilm formation of C. albicans ATCC 10231 cells
Dane BadawczeThe datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.
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Effect of C-1748 derivatives on the morphological transformation of C. albicans ATCC 10231 cells
Dane BadawczeThe datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on morphological transformation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.
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Inserts amplification for knockout cassette construction
Dane BadawczeThe goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the...
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Overproduction of CaMet15p native and His-tag versions.
Dane BadawczeEnzyme of fungal L-methionine biosynthetic pathway: O-acetylhomoserine sulfhydrylase (Met15p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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DES - polarity, pH and antioxidant potential
Dane BadawczeThis physicochemical properties of selected deep eutectic solvents (DES) were tested. Polarity is important for extraction efficiency. The values of pH can importantly affect growing of bacteria and yeasts strains. Total phenolic content, DPPH and FRAP methods were used for determination of antioxidant potential of the extract produced with DES.
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Antifungal activity of propolis extracts produced with deep eutectic solvents.
Dane BadawczeThis dataset contains results of our investigation aiming in determination of antimicrobial potential of the propolis extracts produced with deep eutectic solvents. The activity was determined against C. albicans and C. glabarat strains. On the basis of these results MIC values can be calculated. Three samples of propolis were tested.
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MIC/MBC values of the investigated extracts of propolis. Interactions of selected extracts with azoles and echinocandins.
Dane BadawczeThis dataset contains results of our investigation aiming in determination of antimicrobial potential of the propolis extracts produced in our laboratory. The propolis samples were delivered by Polish beekeepers. Moreover in this dataset we presented results of our investigation aiming in assessment of interaction of the selected extracts of propolis...
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This dataset contains results of our investigation aiming in determination of antioxidant potential of the propolis extract produced in our laboratory.
Dane BadawczeThis dataset contains results of our investigation aiming in determination of antioxidant potential of the propolis extract produced in our laboratory. The propolis samples were delivered by Polish beekeepers. DPPH and FREAP methods were used. Moreover, total phenolic contents in the extracts were determined.
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The impact of QDgreen, QDgreen−β−CD−FA, β−CD, C−2028, β−CD(C−2028), QDgreen−C−2028, and QDgreen−β−CD(C−2028)−FA nanoconjugates on lysosomal content in the cancer (H460, Du-145, LNCaP) and normal (MRC-5, PNT1A) cells
Dane BadawczeThe impact of QDgreen, QDgreen−β−CD−FA, β−CD, C−2028, β−CD(C−2028), QDgreen−C−2028, and QDgreen−β−CD(C−2028)−FA nanoconjugates on lysosomal content in the cancer (H460, Du-145, LNCaP) and normal (MRC-5, PNT1A) cells was performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). To explore the influence...
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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity
Dane BadawczeInhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed...
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Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
Dane BadawczeEnzymes of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p), O-acetylhomoserine sulfhydrylase (Met15p) and cystathionine-γ-synthase (Str2p) could be exploited as molecular targets for antifungal chemotherapy. The goal of the study was to identify and clone genes encoding mentioned above enzymes. MET2, MET15 and STR2 genes...
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The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer H460 cells
Dane BadawczeThe time-dependent (1, 24, 48, and 72 h) cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer H460 cells performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). Based on the fluorescence properties of these compounds, green and orange fluorescence...
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The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer Du-145 cells
Dane BadawczeThe time-dependent (1, 24, 48, and 72 h) cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer Du-145 cells performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). Based on the fluorescence properties of these compounds, green and orange fluorescence...
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The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal PNT1A cells
Dane BadawczeThe time-dependent (1, 24, 48, and 72 h) cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal PNT1A cells performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). Based on the fluorescence properties of these compounds, green and orange fluorescence...
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The mechanism of internalization study of QDgreen−CD−FA−C−2028 conjugate at IC80 value to cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells
Dane BadawczeThe influence of different endocytosis inhibitors on the internalization of QDgreen−CD−FA−C−2028 conjugate at IC80 value in cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells. First, the cells were preincubated with: drug-free medium (no inhibitor), at 4 °C, 5 µM Cytochalasin D, 30 µM Amiloride, 80 µM Dynasore, 25 µM Pitstop 2 and 1.5...
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The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal MRC-5 cells
Dane BadawczeThe time-dependent (1, 24, 48, and 72 h) cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to normal MRC-5 cells performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). Based on the fluorescence properties of these compounds, green and orange fluorescence...
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The time-dependent cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer LNCaP cells
Dane BadawczeThe time-dependent (1, 24, 48, and 72 h) cellular uptake of C−2028, CD−C−2028, QDgreen−C−2028, and QDgreen−CD−FA−C−2028 conjugates at IC80 value to cancer LNCaP cells performed by Confocal Laser Scanning Microscopy (63× magnification; ZEISS LSM T-PMT, Magdeburg, Germany). Based on the fluorescence properties of these compounds, green and orange fluorescence...
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Determination of the minimum inhibitory concentration of C-1311 derivatives (C-1296, C-1410, Compound 1, Compound 1-R8) against Candida strains
Dane BadawczeThe datasets contain the results of determining the minimum inhibitory concentration of imidazoacridinone derivatives against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and C. albicans clinical strains by the modified M27-A3 specified by the CLSI.
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Determination of the minimum inhibitory concentration of C-1305 derivatives (IKE1-IKE8) against Candida strains
Dane BadawczeThe datasets contain the results of determining the minimum inhibitory concentration of acridone derivatives against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 by the modified M27-A3 specified by the CLSI.
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The absorption and fluorescence spectra of C-1305 derivatives (IKE1-IKE8), potential antifungal agents
Dane BadawczeOptical measurements of C-1305 derivatives (IKE1-IKE8). The absorption spectra were recorded at 300 - 800 nm for solutions with 16 μg / mL derivative concentration. The fluorescence emission spectra were determined at 420-800 nm with the excitation wavelengths 360 or 415 nm for solutions with 1 μg / mL derivative concentration. All measurements were...
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The influence of chitosan hydrogels on the morphology of L929 cells
Dane BadawczeRepresentative image at 20x magnification of L929 cells following 24 h of treatment with chitosan derivatives: chitosan dissolved in carbonic acid, chitosan dissolved in carbonic acid endotoxin free, chitosan dissolved in hydroacetic acid at 1:3 diluent factor. The scale bar is 100 µm; Microscope Olympus IX83, Tokyo, Japan).
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Cytotoxicity of chitosan hydrogels aginst L929 cells
Dane BadawczeThis study presents absorbance values of formazan product (converted from MTT) which corresponds to the cytotoxicity of chitosan compounds (dissolved in carbonic acid, dissolved in carbonic acid endotoxin free, and dissolved in hydroxyacetic acid) aginst adult mouse fibroblast L929 cells. The experiments were carried out according to the ISO 10993-5:2009(E)...
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Cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst MRC-5 normal cells
Dane BadawczeTis study presents absorbance values of formazan product (converted from MTT) which corresponds the cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst MRC-5 normal cells. FA (folic acid) with cyclodextrin (CD) was used as a linker between quantum dots (QDs) and compound (C-2028).
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Cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst H460 cancer cells
Dane BadawczeThis study presents absorbance values of formazan product (converted from MTT) which corresponds the cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst H460 cancer cells. FA (folic acid) with cyclodextrin (CD) was used as a linker between quantum dots (QDs) and compound (C-2028).
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Cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst LNCaP cancer cells
Dane BadawczeTis study presents absorbance values of formazan product (converted from MTT) which corresponds the cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst LNCaP cancer cells. FA (folic acid) with cyclodextrin (CD) was used as a linker between quantum dots (QDs) and compound (C-2028).
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Cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst Du-145 cancer cells
Dane BadawczeTis study presents absorbance values of formazan product (converted from MTT) which corresponds the cytotoxicity of C-2028, QDgreen, QDred, QDgreen-CD-FA, QDred-CD-FA, QDgreen-CD-FA-C-2028 and QDred-CD-FA-C-2028 aginst LNCaP cancer cells. FA (folic acid) with cyclodextrin (CD) was used as a linker between quantum dots (QDs) and compound (C-2028).
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Involvement of human glutathione S-transferase M1-1 in the glutathione conjugation of the antitumor unsymmetrical bisacridine derivative C-2028.
Dane BadawczeUnsymmetrical bisacridines (UAs) are a novel potent class of antitumor-active therapeutics. The aim of this study was to investigate the possible role of human glutathione S-transferase M1-1 (hGSTM1-1) in the glutathione (GSH) conjugation of a representative UA, C‑2028.
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Determination the method of free alcohol fermented beverage production based on roasted raw materials.
Dane BadawczeData set presents the results of monosaccharides and ethyl alcohol determination in samples of fermented cereal drink with increased health value based on barley malt and roasting of rye, chicory and beetroot, and with the addition of hops. The HPLC method with RID detector was used for determinations