Aleksandra Steć - Publications - Bridge of Knowledge

Aleksandra Steć

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Year 2022

Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis
Publication
- A. Steć
- J. Jońca
- K. Waleron
- M. Waleron
- A. Płoska
- L. Kalinowski
- B. Wielgomas
- S. Dziomba
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2022
Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory...

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