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Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A
Description
Bacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids, agar, and yeast extract. The fluorescent dye specifically attaches to cellulose, enabling bacterial colonies to be observed under ultraviolet light. The study employed YESCA medium supplemented with calcofluor-white, as well as YESCA + 0.2% glucose medium supplemented with calcofluor-white.
The dataset contains original photos of bacterial colonies of the following E. coli strains: BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP AAEC191A/pCC90, AAEC191A/pACYCpBAD, AAEC191A/pCC90 Dra D-mut, AAEC191A/pCC90 D54-STOP, BL21(DE3), AAEC191A and CFT073. Bacteria were cultured in YESCA medium with the addition of calcofluor-white at a temperature of 25OC and 37OC for 5 days. Photographs of the colony were taken at one-day intervals.
Dataset file
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.Example script for calculation:
https://github.com/antespi/s3md5
File details
- License:
-
open in new tab
CC BYAttribution - File embargo:
- 2024-06-30
Details
- Year of publication:
- 2023
- Verification date:
- 2024-01-03
- Creation date:
- 2023
- Dataset language:
- English
- Fields of science:
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- biological sciences (Natural sciences)
- chemical sciences (Natural sciences)
- DOI:
- DOI ID 10.34808/zgfw-7g12 open in new tab
- Funding:
- Series:
- Verified by:
- Gdańsk University of Technology
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