Detection of UPEC IH11128 ability to form biofilm in a standard congo red (CR) method of colony staining. - Open Research Data - Bridge of Knowledge

Search

Detection of UPEC IH11128 ability to form biofilm in a standard congo red (CR) method of colony staining.

Description

A standard method of testing E. coli's ability to form biofilm is based on the analysis of morphology
of bacterial colonies grown on yeast extract/casamino acids (YESCA) nutrient agar medium containing congo
red (CR) as an indicator dye of ECM production. The CR dye adsorbed from the culture medium by
bacteria binds to the components of the ECM, including curli and cellulose, giving the bacterial colonies a
dark red color.

The dataset contains original photos of bacterial colonies of the following E. coli strains: IH11128, DR14, CFT073, AAEC191A/pCC90 and AAEC191A/pACYC184. Bacteria were cultured on a YESCA medium at 25 degrees Celsius for 5 days. Pictures of the colonies were taken at intervals of one day.

Dataset file

Congo Red-experiment.zip
196.9 MB, S3 ETag d6e5bd0540974cb2d2c128b0984e8fca-1, downloads: 21
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5
download file Congo Red-experiment.zip

File details

License:
Creative Commons: 0 1.0 open in new tab
CC 0
Public Domain Dedication
Raw data:
Data contained in dataset was not processed.

Details

Year of publication:
2023
Verification date:
2023-03-16
Creation date:
2022
Dataset language:
English
Fields of science:
  • Biological sciences (Natural sciences)
  • Chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/qh3q-4m93 open in new tab
Funding:
Verified by:
Gdańsk University of Technology

Keywords

Cite as

seen 72 times