Study of the effect of Dr fimbria presence on the accumulation of recombinant Escherichia coli strain: AAEC191A/pCC90 cells in polystyrene in a dynamic system - Open Research Data - Bridge of Knowledge

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Study of the effect of Dr fimbria presence on the accumulation of recombinant Escherichia coli strain: AAEC191A/pCC90 cells in polystyrene in a dynamic system

Description

The process of surface colonization, known as biofilm development, begins with bacterial attachment and involves various physicochemical and molecular interactions. Adhesion to neutral surfaces typically involves non-specific interactions, while adhesion to biological surfaces is governed by specific ligand-receptor interactions. In the experiment, the accumulation of recombinant Escherichia coli strains (AAEC191A/pCC90 and AAEC191A/pBAD) was studied under continuous flow conditions on glass and polystyrene surfaces. The objective was to assess how Dr fimbriae influenced bacterial accumulation on different surfaces. Bacterial cells were suspended in a phosphate buffer solution with a pH of 5.7, and the flow rate of the solution was tested at three levels: 0.02 ml/min, 0.1 ml/min, and 0.5 ml/min, with each flow lasting 15 minutes.

The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

Dataset file

AAEC191A pCC90 (PBS pH=5,7)- POLYSTYRENE.zip
103.1 GB, S3 ETag 62608cc7f9132df23ef3b03ad5bec96b-207, downloads: 0
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File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
File embargo:
2025-06-30
Raw data:
Data contained in dataset was not processed.
Software:
cellSens Olympus

Details

Year of publication:
2024
Verification date:
2024-12-23
Dataset language:
English
Fields of science:
  • chemical sciences (Natural sciences)
  • biological sciences (Natural sciences)
DOI:
DOI ID 10.34808/er0v-3k75 open in new tab
Funding:
Verified by:
Gdańsk University of Technology

Keywords

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