Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: AAEC191A/pCC90 to glass in a dynamic system - PBS pH=8 - Open Research Data - Bridge of Knowledge

Search

Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: AAEC191A/pCC90 to glass in a dynamic system - PBS pH=8

Description

The attachment of bacteria begins the process of surface colonization, called biofilm development, characterized by a number of physicochemical and molecular interactions. Adherence to inert surfaces typically involves nonspecific interactions, whereas adherence to biological surfaces is associated with specific ligand-receptor interactions. In this study, the adherence ability of two genetically modified Escherichia coli strains— AAEC191A/pCC90, and AAEC191A/pACYCpBAD—was examined on both glass and polystyrene surfaces. The aim was to investigate the effect of the presence of Dr fimbriae on the adherence of bacteria to various carriers. The experiments were performed under various flow conditions. cells were suspended in phosphate buffers of different pH=5.7, pH=7 and pH=8

The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

Dataset file

AAEC191A pCC90 (PBS pH=8)- GLASS.zip
42.0 GB, S3 ETag 2fc7263b6d3fe0a14b09d02f3e3ec3b0-84, downloads: 0
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
File embargo:
2025-06-30
Raw data:
Data contained in dataset was not processed.
Software:
cellSens Olympus

Details

Year of publication:
2024
Verification date:
2024-12-23
Dataset language:
English
Fields of science:
  • chemical sciences (Natural sciences)
  • biological sciences (Natural sciences)
DOI:
DOI ID 10.34808/8444-0f82 open in new tab
Funding:
Series:
Verified by:
Gdańsk University of Technology

Keywords

Cite as

seen 14 times