Study of the influence of the presence of Dr fimbriae on the sedimentation of recombinant Escherichia coli strains: AAEC191A and BL21(DE3)

Description

Cell sedimentation in the medium is a common phenomenon in most bacterial enviroments. This study specifically investigated the impact of Dr fimbriae presence on cell deposition. To explore this, recombinant Escherichia coli strains were employed, including BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP, AAEC191A/pCC90, AAEC191A/pACYCpBAD, AAEC191A/pCC90 Dra D-mut, and AAEC191A/pCC90 D54-STOP. Spectroscopic methods were utilized for the measurements.

The dataset contains original photos of test tubes containing overnight cultures of the above-mentioned recombinant strains and a Word document with graphs showing the time dependence of cell precipitation.

Dataset file

Cell sedimentation over time - experiment.zip

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CC BY

Attribution
File embargo:: 2024-06-30

Details

Year of publication:

2023

Verification date:

2024-01-03

Creation date:

2023

Dataset language:

English

Fields of science:

biological sciences (Natural sciences)
chemical sciences (Natural sciences)

DOI:

10.34808/w3jn-h127

Funding:

Mechanism of biofilm formation by uropatogenic E. coli producing fimbrial adhesin Dr - studies of proteome and metabolome changes under static and dynamic culture conditions

Verified by:

Gdańsk University of Technology

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