The database of localization and expression of aquaporin 3 (AQP3), aquaporin 7 (AQP7) and aquaporin 9 (AQP9) in the male reproductive system in cattle. Morphometric studies. Localization of zinc finger transcription factor GATA-4. - Open Research Data - Bridge of Knowledge

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The database of localization and expression of aquaporin 3 (AQP3), aquaporin 7 (AQP7) and aquaporin 9 (AQP9) in the male reproductive system in cattle. Morphometric studies. Localization of zinc finger transcription factor GATA-4.

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Description

The data present research results being part of the OPUS-22 project entitled “In search of new markers of male fertility in cattle. Aquaporins expression in the reproductive organs and sperm of the bulls (Bos taurus)” obtained from the National Science Center in Poland (grant no. 2021/43/B/NZ9/00204). The aim of this part of the project was (i) to determine the location of aquaglyceroporins (AQP3, AQP7 and AQP9) in the male reproductive system - from the testis to the vas deferens in cattle and (ii) to analyze changes in the expression of these proteins during animal growth and development, (iii) to assess the morphology of bovine male reproductive tract, (iv) to identify the Sertoli and Leydig cells using GATA-4 antibodies.

This dataset contains:

  • raw data from the measurement of body weight, right testis and epididymis weights;
  • raw data from morphometric studies;
  • microphotographs of immunohistochemical staining of GATA-4, AQP3, AQP7 and AQP9;
  • raw data from the analyses of AQPs expression, Western blot images and blots measured in Image Lab software.

All necessary information regarding the above data and a kind of instruction for viewing them are included in the file READ ME.docx.

Dataset file

DATA1.zip
3.9 GB, S3 ETag d2714cbbd6ccc9408787b10ef1a7999b-8, downloads: 55
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5
download file DATA1.zip

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
Raw data:
Data contained in dataset was not processed.
Software:
Image Lab, Statistica

Details

Year of publication:
2023
Verification date:
2023-12-14
Creation date:
2023
Dataset language:
English
Fields of science:
  • animal science and fisheries (Agricultural sciences)
DOI:
DOI ID 10.34808/j254-s011 open in new tab
Funding:
Ethical papers:
Approval no. 243/2019 by II Lokalna Komisja Etyczna do Spraw Doświadczeń na Zwierzętach w Krakowie
Verified by:
West Pomeranian University of Technology in Szczecin

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Version this document has several versions

  • Current version
    version 1.0
    DOI ID10.34808/j254-s011
    release date 2023-12-14
DOI 10.34808/sk5p-bg67 represents the latest version of the data.

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