The estrogenicity of wastewater samples.

Description

The estrogenicity of influent and effluent waters samples from wastewater treatment plant was done by in vivo Estrogen Receptor PolarScreenTM-α Competitor Assay.

The principle of operation of the kit is based on the fact that the tested sample with endocrine activity (containing compounds capable of binding to the human estrogen receptor α, hERα) releases the fluorescently labeled ligand (FluormoneTM ES2) from the receptor-ligand complex, which results in a reduction of the polarization value fluorescence. The maximum value of fluorescence polarization is shown by the receptor-ligand complex, while the minimum mixture containing additionally 1 µM 17β-estradiol (the sex hormone with the highest biological activity).
The endocrine potential of the tested samples was compared with the activity of estradiol by determining the endocrine equivalent concentration (EEQ).

Dataset file

ER.zip

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CC BY-NC-SA

Non-commercial - Share-alike
Software:: EXEL

Details

Year of publication:

2021

Verification date:

2021-04-30

Dataset language:

English

Fields of science:

chemical sciences (Natural sciences)

DOI:

10.34808/1edn-de33

Verified by:

Gdańsk University of Technology

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