Search results for: psychrophilic bacteria

• Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins

  Publication

  • M. Olszewski
  • J. Kur

  - Year 2010

  To study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...

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• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum

  Publication

  • M. Nowak
  • M. Olszewski
  • M. Skwarecka
  • J. Kur

  - BMC MICROBIOLOGY - Year 2014

  Background: Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination and repair in Bacteria, Archaea and Eukarya. In recent years, there has been an increasing interest in SSBs, since they find numerous applications in diverse molecular biology and analytical methods. Results: We report the characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea...

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• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria

  Publication

  • M. Olszewski
  • M. Nowak
  • M. Skwarecka

  - Acta Biochimica Polonica - Year 2013

  Single-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNAinsequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination.Although there are differences in amino acid...

• Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria

  Publication

  • M. Olszewski
  • M. Nowak
  • M. Skwarecka

  - Acta Biochimica Polonica - Year 2013

  Single-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNA insequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination. Although there are differences in amino...

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• Identification and characterization of single-stranded DNA-binding protein from the facultative psychrophilic bacteria Pseudoalteromonas haloplanktis

  Publication

  • M. Olszewski
  • M. Nowak
  • A. Cyranka-Czaja
  • J. Kur

  - MICROBIOLOGICAL RESEARCH - Year 2014

  Single-stranded DNA-binding protein (SSB) plays an important role in DNA metabolism such as DNAreplication, repair, and recombination, and is essential for cell survival. This study reports on the ssb-likegene cloning, gene expression and characterization of a single-stranded DNA-binding protein of Pseudoal-teromonas haloplanktis (PhaSSB) and is the first report of such a protein from psychrophilic microorganism.PhaSSB possesses...

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• Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus

  Publication

  • M. Nowak
  • M. (. Olszewski
  • J. Kur

  - Acta Biochimica Polonica - Year 2011

  To study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....

• Novel single-stranded DNA-binding proteins from extreme psychrophilic bacterium Psychromonas ingrahamii 37

  Publication

  • M. Olszewski
  • M. Nowak
  • N. Maciejewska
  • J. Kur

  - Year 2010

  We report the identification and characterization of the single-stranded DNA binding protein (SSB) from extreme psychrophilic bacterium Psychromonas ingrahamii 37 (PinSSB) that grows expotentially at -12°C and may well grow at even lower temperatures. PinSSB is one of the largest known bacterial SSB protein consisting 222 amino acid residues with a calculated molecular mass of 25.1 kDa. The analysis by gel filtration chromatography...

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• Molecular modelling of a psychrophilic β-galactosidase

  Publication

  • K. Tkaczuk
  • J. M. Bujnicki
  • A. Białkowska
  • S. Bielecki
  • M. Turkiewicz
  • H. Cieśliński
  • J. Kur

  - Year 2005

  Antarktyczny szczep, gram-ujemnych bakterii, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, sklasyfikowany na podstawie analizy sekwencji nukleotydowej geny 16S rDNA jako Pseudoalteromonas sp. 22b jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Ponieważ enzym ten wykazuje wysoką aktywność katalityczną w zakresie temperatur od 0 do 30 stC, stanowi on atrakcyjny biokatalizator, użyteczny...

• Materia

  Journals

  ISSN: 1579-2641 , eISSN: 2385-3387

• Materia Arquitectura

  Journals

  ISSN: 0718-7033