New methods for separation, isolation and characterisation of extracellular vesicles based on the two-dimensional high-performance liquid chromatography and mass spectrometry techniques - Project - Bridge of Knowledge

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New methods for separation, isolation and characterisation of extracellular vesicles based on the two-dimensional high-performance liquid chromatography and mass spectrometry techniques

This project aims to explore the possibilities arising from the application of different modes of one- and two-dimensional high-performance liquid chromatography (HPLC) in EVs isolation and fractionation. Robust and efficient high-performance liquid chromatography techniques especially when directly coupled to mass spectrometry can accelerate basic research and quality control of drug formulations containing EVs. Within the frame of the project it is planned to i) develop a new HPLC method based on hydrophobic interaction chromatography (HIC) mode for effective isolation and separation of EV subpopulations; ii) develop two-dimensional HPLC methods involving two of three orthogonal separation modes based on hydrophobicity, size and ionic properties for comprehensive separation of EV fractions; iii) develop LC-Q-TOF method to study lipid composition of the EV fractions. For the development of the method, human serum EVs and Cyanobacterial EVs will be used as biological samples. EV subpopulation fractions will be characterized with state-of-the-art techniques such as electron microscopy, western blot and nanoparticle tracking analysis by expertise collaborators. Research planned in this project requires collaboration within multidisciplinary team comprised of the international scientists: - Prof. Dr Sebastiaan Eeltink, Department of Chemical Engineering at the Vrije University of Brussels, dr Paulo Oliveira from the Institute for Research and Innovation in Health, University of Porto and dr hab. Danuta Gutowska-Owsiak, prof at the University of Gdańsk, the head of the Laboratory of Experimental and Translational Immunology Group, Institute of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk.

Details

Financial Program Name:
SONATA
Organization:
Narodowe Centrum Nauki (NCN) (National Science Centre)
Agreement:
UMO-2021/43/D/ST4/02872 z dnia 2022-07-04
Realisation period:
2022-07-04 - 2025-07-03
Project manager:
dr hab. inż. Weronika Hewelt-Belka
Team members:
Realised in:
Department of Analytical Chemistry
Project's value:
1 431 000.00 PLN
Request type:
National Research Programmes
Domestic:
Domestic project
Verified by:
Gdańsk University of Technology

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