A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis - Publication - Bridge of Knowledge

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A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis

Abstract

The preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis was suspected was higher, at 100% and 95.4%, respectively, than for people with chronic infection, at 88.2% and 70.6%. In this study a different trend in avidity maturation of IgG antibodies for two mixtures of proteins in comparison with native antigen was observed. The results suggest that a new IgG avidity test using the mixtures of recombinant antigens may be useful for the diagnosis of difficult-to-identify phases of toxoplasmosis. For this reason, selected mixtures after the additional tests on groups of sera with well-defined dates of infection could be used as a better alternative to the native antigens of the parasite in the serodiagnosis of human T. gondii infection.

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Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE no. 79, edition 3, pages 342 - 346,
ISSN: 0732-8893
Language:
English
Publication year:
2014
Bibliographic description:
Drapała D., Holec-Gąsior L., Kur J., Ferra B., Hiszczyńska-Sawicka E., Lautenbach D.: A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis// DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. -Vol. 79, iss. 3 (2014), s.342-346
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.diagmicrobio.2014.03.005
Verified by:
Gdańsk University of Technology

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