Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Aneta Łuczkiewicz; Ewa Kotlarska; Wojciech Artichowicz; Katarzyna Tarasewicz; Sylwia Fudala-Książek

doi:10.1007/s11356-015-5098-y

Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone

Abstract

In this study, species distribution and antimicrobial susceptibility of cultivated Pseudomonas spp. were studied in influent (INF), effluent (EFF), and marine outfall (MOut) of wastewater treatment plant (WWTP). The susceptibility was tested against 8 antimicrobial classes, active against Pseudomonas spp.: aminoglycosides, carbapenems, broad-spectrum cephalosporins from the 3rd and 4th generation, extended-spectrum penicillins, as well as their combination with the β-lactamase inhibitors, monobactams, fluoroquinolones, and polymyxins. Among identified species, resistance to all antimicrobials but colistin was shown by Pseudomonas putida, the predominant species in all sampling points. In other species, resistance was observed mainly against ceftazidime, ticarcillin, ticarcillin-clavulanate, and aztreonam, although some isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas pseudoalcaligenes, and Pseudomonas protegens showed multidrug-resistance (MDR) phenotype. Among P. putida, resistance to β-lactams and to fluoroquinolones as well as multidrug resistance become more prevalent after wastewater treatment, but the resistance rate decreased in marine water samples. Obtained data, however, suggests that Pseudomonas spp. are equipped or are able to acquire a wide range of antibiotic resistance mechanisms, and thus should be monitored as possible source of resistance genes.

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Authors (5)

Aneta Łuczkiewicz prof. dr hab. inż.
Ewa Kotlarska
- Instytut Oceanologii PAN Pracownia Biologii Molekularnej
Wojciech Artichowicz dr inż.
Katarzyna Tarasewicz mgr
Sylwia Fudala-Książek dr hab. inż.

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Keywords

PSEUDOMONAS SPP. SPECIES DISTRIBUTION ANTIMICROBIAL SUSCEPTIBILITY WASTEWATER MARINE OUTFALL

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH no. 22, edition 24, pages 1 - 12,
ISSN: 0944-1344
Language:: English
Publication year:: 2015
Bibliographic description:: Łuczkiewicz A., Kotlarska E., Artichowicz W., Tarasewicz K., Fudala-Książek S.: Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone// ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH. -Vol. 22, iss. 24 (2015), s.1-12
DOI:: Digital Object Identifier (open in new tab) 10.1007/s11356-015-5098-y
Verified by:: Gdańsk University of Technology