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INFOGEST static in vitro simulation of gastrointestinal food digestion

Abstract

Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.

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Authors (35)

  • Photo of  André Brodkorb

    André Brodkorb

    • -
  • Photo of  Lotti Egger

    Lotti Egger

    • -
  • Photo of  Marie Alminger

    Marie Alminger

    • -
  • Photo of  Paula Alvito

    Paula Alvito

    • -
  • Photo of  Ricardo Assunção

    Ricardo Assunção

    • -
  • Photo of  Simon Ballance

    Simon Ballance

    • -
  • Photo of  Torsten Bohn

    Torsten Bohn

    • -
  • Photo of  Claire Bourlieu-Lacanal

    Claire Bourlieu-Lacanal

    • -
  • Photo of  Rachel Boutrou

    Rachel Boutrou

    • -
  • Photo of  Frédéric Carrière

    Frédéric Carrière

    • -
  • Photo of  Alfonso Clemente

    Alfonso Clemente

    • -
  • Photo of  Milena Corredig

    Milena Corredig

    • -
  • Photo of  Didier Dupont

    Didier Dupont

    • -
  • Photo of  Claire Dufour

    Claire Dufour

    • -
  • Photo of  Cathrina Edwards

    Cathrina Edwards

    • -
  • Photo of  Matt Golding

    Matt Golding

    • -
  • Photo of  Sibel Karakaya

    Sibel Karakaya

    • -
  • Photo of  Bente Kirkhus

    Bente Kirkhus

    • -
  • Photo of  Steven Le

    Steven Le

    • -
  • Photo of  Uri Lesmes

    Uri Lesmes

  • Photo of  Alan Mackie

    Alan Mackie

    • -
  • Photo of  Carla Martins

    Carla Martins

    • -
  • Photo of  Sébastien Marze

    Sébastien Marze

    • -
  • Photo of  David McClements

    David McClements

    • -
  • Photo of  Olivia Ménard

    Olivia Ménard

    • -
  • Photo of  Mans Minekus

    Mans Minekus

    • -
  • Photo of  Reto Portmann

    Reto Portmann

    • -
  • Photo of  Cláudia Santos

    Cláudia Santos

    • -
  • Photo of  Isabelle Souchon

    Isabelle Souchon

    • -
  • Photo of  R Singh

    R Singh

    • -
  • Photo of  Gerd Vegarud

    Gerd Vegarud

    • -
  • Photo of  Martin Wickham

    Martin Wickham

    • -
  • Photo of  Werner Weitschies

    Werner Weitschies

    • -
  • Photo of  Isidra Recio

    Isidra Recio

    • -

Cite as

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DOI:
Digital Object Identifier (open in new tab) 10.1038/s41596-018-0119-1
License
Copyright (2019 Springer Nature America, Inc.)

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
Nature Protocols no. 14, pages 991 - 1014,
ISSN: 1754-2189
Language:
English
Publication year:
2019
Bibliographic description:
Brodkorb A., Egger L., Alminger M., Alvito P., Assunção R., Ballance S., Bohn T., Bourlieu-Lacanal C., Boutrou R., Carrière F., Clemente A., Corredig M., Dupont D., Dufour C., Edwards C., Golding M., Karakaya S., Kirkhus B., Le S., Lesmes U., Macierzanka A., Mackie A., Martins C., Marze S., Mcclements D., Ménard O., Minekus M., Portmann R., Santos C., Souchon I., Singh R., Vegarud G., Wickham M., Weitschies W., Recio I.: INFOGEST static in vitro simulation of gastrointestinal food digestion// Nature Protocols. -Vol. 14, (2019), s.991-1014
DOI:
Digital Object Identifier (open in new tab) 10.1038/s41596-018-0119-1
Verified by:
Gdańsk University of Technology

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