Mechanisms of azole resistance among clinical isolates of Candida glabrata in Poland

Piotr Szweda; Katarzyna Magdalena Gucwa; Ewa Romanowska; Katarzyna Dzierżanowska-Fangrat; Łukasz Naumiuk; Anna Brillowska-Dąbrowska; Iwona Wojciechowska-Koszko; Sławomir Milewski

doi:10.1099/jmm.0.000062

Mechanisms of azole resistance among clinical isolates of Candida glabrata in Poland

Abstract

Candida glabrata is currently ranked as the second most frequently isolated aetiological agent of human fungal infections, next only to Candida albicans. In comparison with C. albicans, C. glabrata shows lower susceptibility to azoles, the most common agents used in treatment of fungal infections. Interestingly, the mechanisms of resistance to azole agents in C. albicans have been much better investigated than those in C. glabrata. The aim of the presented study was to determine the mechanisms of resistance to azoles in 81 C. glabrata clinical isolates from three different hospitals in Poland. The investigation was carried out with a Sensititre Yeast One test and revealed that 18 strains were resistant to fluconazole, and 15 were cross-resistant to all other azoles tested (voriconazole, posaconazole and itraconazole). One isolate resistant to fluconazole was cross-resistant to voriconazole, and resistance to voriconazole only was observed in six other isolates. All strains were found to be susceptible to echinocandins and amphotericin B, and five were classified as resistant to 5-fluorocytosine. The sequence of the ERG11 gene encoding lanosterol 14-a demethylase (the molecular target of azoles) of 41 isolates, including all strains resistant to fluconazole and three resistant only to voriconazole, was determined, and no amino acid substitutions were found. Real-time PCR studies revealed that 13 of 15 azole-resistant strains showed upregulation of the CDR1 gene encoding the efflux pump. No upregulation of expression of the CDR2 or ERG11 gene was observed.

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Authors (8)

Piotr Szweda dr hab. inż.
Katarzyna Magdalena Gucwa
Ewa Romanowska
- Szpital-Pomnik Centrum Zdrowia Dziecka Katedra Mikrobiologii Klinicznej i Immunologii
Katarzyna Dzierżanowska-Fangrat
- Szpital-Pomnik Centrum Zdrowia Dziecka Katedra Mikrobiologii Klinicznej i Immunologii
Łukasz Naumiuk
- Uniwersyteckie Centrum Kliniczne GUMed Katedra Mikrobiologii Klinicznej
Anna Brillowska-Dąbrowska dr hab. inż.
Iwona Wojciechowska-Koszko
- Pomorski Uniwersytet Medyczny Katedra Mikrobiologii i Immunologii
Sławomir Milewski prof. dr hab. inż.

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DOI:: Digital Object Identifier (open in new tab) 10.1099/jmm.0.000062
License: Copyright (2015 Microbiology Society)

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF MEDICAL MICROBIOLOGY no. 64, edition 6, pages 610 - 619,
ISSN: 0022-2615
Language:: English
Publication year:: 2015
Bibliographic description:: Szweda P., Gucwa K., Romanowska E., Dzierżanowska-Fangrat K., Naumiuk Ł., Brillowska-Dąbrowska A., Wojciechowska-Koszko I., Milewski S.: Mechanisms of azole resistance among clinical isolates of Candida glabrata in Poland// JOURNAL OF MEDICAL MICROBIOLOGY. -Vol. 64, iss. 6 (2015), s.610-619
DOI:: Digital Object Identifier (open in new tab) 10.1099/jmm.0.000062
Verified by:: Gdańsk University of Technology