Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines - Publication - Bridge of Knowledge

Search

Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines

Abstract

Epidemiological studies have shown that consumption of cab- bage and sauerkraut is connected with significant reduction of breast cancer incidences. Estrogens are considered a major breast cancer risk factor and their metabolism by P450 enzymes substan- tially contributes to carcinogenic activity. The aim of this study was to investigate the effect of cabbage and sauerkraut juices of differ- ent origin on the expression profile of the estrogen metabolism key enzymes (CYP1A1, CYP1A2, CYP1B1) in breast cell lines MCF7, MDA-MB-231, and MCF10A. The effects of cabbage juices were compared with that exerted by indole-3-carbinol (I3C) and 3,3'- diindolylmethane (DIM). The treatment with cabbage juices or indoles for 72 h affected the expression of CYP1 family genes in cell-type dependent manner. Their induction was found in all cell lines, but the ratio of CYP1A1 to CYP1B1 was 1.22- to 10.6-fold in favor to CYP1A1 in MCF7 and MCF10A cells. Increased lev- els of CYP1A2 in comparison with CYP1B1 were also observed in MCF7 cells. In contrast, in MDA-MB-231 cells CYP1B1 was preferentially induced. Since the cell lines investigated differ in in- vasion capacity, these results support epidemiological observations and partly explain the mechanism of the chemopreventive activity of white cabbage products.

Citations

  • 2 4

    CrossRef

  • 0

    Web of Science

  • 2 6

    Scopus

Cite as

Full text

full text is not available in portal

Keywords

Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL no. 64, pages 879 - 888,
ISSN: 0163-5581
Language:
English
Publication year:
2012
Bibliographic description:
Szaefer H., Licznerska B., Krajka-Kuźniak V., Bartoszek-Pączkowska A., Baer-Dubowska W.: Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines// NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL. -Vol. 64, nr. iss. 6 (2012), s.879-888
DOI:
Digital Object Identifier (open in new tab) 10.1080/01635581.2012.690928
Verified by:
Gdańsk University of Technology

seen 108 times

Recommended for you

Meta Tags