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Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood–brain barrier
Abstract
The blood–brain barrier (BBB) tightly controls the microenvironment of the central nervous system (CNS) to allow neurons to function properly. Additionally, emerging studies point to the beneficial effect of natural oils affecting a wide variety of physiological and pathological processes in the human body. In this study, using an in vitro model of the BBB, we tested the influence of natural fish oil mixture (FOM) vs. borage oil (BO), both rich in longchain polyunsaturated fatty acids (LC-PUFAs) and monounsaturated fatty acids (MUFAs) such as oleic acid (C18:1n9c) or nervonic acid (NA), on human oligodendrocyte precursor cells (hOPCs) during their maturation to oligodendrocytes (OLs) regarding their ability to synthesize myelin peptides and NA. We demonstrated that FOM, opposite to BO, supplemented endothelial cells (ECs) and astrocytes forming the BBB, affecting the function of hOPCs during their maturation. This resulted in improved synthesis of myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), proteolipid protein (PLP), and NA in mature OLs. This effect is probably the result of BBB cell and hOPC stimulation via free fatty acid receptors (FFARs), which increases insulin growth factor-1 (IGF-1), ciliary neurotrophic factor (CNTF), and brainderived neurotrophic factor (BDNF) and inhibits fibroblast growth factor 2 (FGF-2) synthesis. The unique formula of fish oil, characterized by much more varied components compared to those of BOs, also improved the enhancement of the tight junction by increasing the expression of claudin-5 and VE-cadherin on ECs. The obtained data justify consideration of naturally derived fish oil intake in human diet as affecting during remyelination.
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Authors (8)
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Paweł Piatek
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Natalia Lewkowicz
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Sylwia Michlewska
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Marek Wieczorek
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Radosław Bonikowski
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Przemysław Lewkowicz
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Magdalena Namiecinska
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3389/fimmu.2022.932383
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
Frontiers in Immunology
no. 13,
pages 932383 - .,
ISSN: 1664-3224 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Piatek P., Lewkowicz N., Michlewska S., Wieczorek M., Bonikowski R., Parchem K., Lewkowicz P., Namiecinska M.: Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood–brain barrier// Frontiers in Immunology -Vol. 13, (2022), s.932383-.
- DOI:
- Digital Object Identifier (open in new tab) 10.3389/fimmu.2022.932383
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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