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New conjugates of tuftsin and muramyl dipeptide as stimulators of human monocytes-derived dendritic cells
Abstract
Muramyl dipeptide (MDP) and tuftsin are known biologically active compound displaying a significant influence on various cell populations of innate immune response. MDP, as a fragment of bacterial cell wall, stimulates not only macrophages and monocytes, but also dendritic cells. In contrast, little is known about tuftsin influence on these cells. Therefore it seemed vital to access whether tuftsin or its derivatives conjugated with MDP could influence the activity of this subpopulation of antigen presenting cells (APC). Immature dendritic cells (iDCs) were derived from human monocytes through eight-day tissue culture supplemented with hrIL-4 and hrGM-CSF. On the day 9 DCs were stimulated with newly synthesized conjugates of tuftsin and muramyl dipeptide. The influence of the examined compounds on the activity and maturity of monocyte-derived DCs was estimated by flow cytometry analysis. The flow cytometry analysis revealed that tuftsin and some of its analogues do stimulate maturation and activity of DCs but to a lesser extend in comparison to MDP. The obtained results suggest further development of the experiments concerning the influence of MDP and tuftsin analogues on the activity of dendritic cells.
Authors (4)
-
Anna Wardowska
- GUMed Zakład Immunologii i Transplantologii
-
Agnieszka Menderska
- GUMed Zakład Immunologii i Transplantologii
-
Piotr Trzonkowski
- GUMed Zakład Immunologii i Transplantologii
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
PROTEIN AND PEPTIDE LETTERS
no. 20,
edition 2,
pages 200 - 204,
ISSN: 0929-8665 - Language:
- English
- Publication year:
- 2013
- Bibliographic description:
- Wardowska A., Dzierzbicka K., Menderska A., Trzonkowski P.: New conjugates of tuftsin and muramyl dipeptide as stimulators of human monocytes-derived dendritic cells// PROTEIN AND PEPTIDE LETTERS. -Vol. 20, iss. 2 (2013), s.200-204
- Verified by:
- Gdańsk University of Technology
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Conjugates of tuftsin and muramyl dipeptide as stimulators of monocyte-derived dendritic cells
- A. Waradowska,
- K. Dzierzbicka,
- A. Menderska
- + 1 authors