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Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer
Abstract
Mercury, a toxic heavy metal produced through both natural and anthropogenic processes, is found in all of Earth’s major systems. Mercury’s bioaccumulation characteristics in the human body have a significant impact on the liver, kidneys, brain, and muscles. In order to detect Hg2+ ions, a highly sensitive and specific fluorescent biosensor has been developed using a novel, modified seven amino acid peptide, FY7. The tyrosine ring in the FY7 peptide sequence forms a 2:1 complex with Hg2+ ions that are present in the water-based sample. As a result, the peptide’s fluorescence emission decreases with higher concentrations of Hg2+. The FY7 peptide’s performance was tested in the presence of Hg2+ ions and other metal ions, revealing its sensitivity and stability despite high concentrations. Conformational changes to the FY7 structure were confirmed by FTIR studies. Simultaneously, we designed a miniaturized setup to support an in-house-developed micro-volume capillary container for volume fluorometry measurements. We compared and verified the results from the micro-volume system with those from the commercial setup. The micro-volume capillary system accommodated only 2.9 µL of sample volume, allowing for rapid, sensitive, and selective detection of toxic mercury (II) ions as low as 0.02 µM.
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Authors (8)
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Marta Sosnowska
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Emil Pitula
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Monika Janik
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Mateusz Śmietana
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Marcin Olszewski
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Dawid Nidzworski dr
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Beata Gromadzka
Cite as
Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/bios14110530
- License
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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Biosensors-Basel
no. 14,
ISSN: 2079-6374 - Language:
- English
- Publication year:
- 2024
- Bibliographic description:
- Sosnowska M., Pitula E., Janik M., Bruździak P., Śmietana M., Olszewski M., Nidzworski D., Gromadzka B.: Peptide-Based Rapid and Selective Detection of Mercury in Aqueous Samples with Micro-Volume Glass Capillary Fluorometer// Biosensors-Basel -,iss. 14(11) (2024), s.530-
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/bios14110530
- Sources of funding:
-
- Free publication
- Verified by:
- Gdańsk University of Technology
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