Phage-Directed Synthesis of Photoluminescent Zinc Oxide Nanoparticles under Benign Conditions

Kamila Sadowska; Joanna Karczewska-Golec; Jakub Karczewski; Marcin Łoś; Andrzej Kłonkowski; Grzegorz Węgrzyn; Piotr Golec

doi:10.1021/acs.bioconjchem.6b00196

Phage-Directed Synthesis of Photoluminescent Zinc Oxide Nanoparticles under Benign Conditions

Abstract

Biological systems, especially bacteriophages and peptides, are an attractive green alternative to other known methods of nanoparticle synthesis. In this work, for the first time, bacteriophages were employed to synthesize a specific peptide, capable of producing nanoparticles (NPs). Derivatives of M13 bacteriophage exposing a ZnO-binding peptide (TMGA-LGLKWPV) on either pIII or pVIII phage coat protein were constructed and used as a biotemplate. The exposition of the ZnO-binding peptide, synthesized by phages during their propagation in bacteria, on M13 virions provided a groundwork for growing ZnO nanostructures. Depending on the recombinant phage type used (M13-pIII-ZnO or 13-pVIII-ZnO), well separated ZnO NPs or complex 3D structures of ZnO NPs of ca. 20−40 nm were synthesized at room temperature. The synthesized ZnO nanoparticles served as a luminescent material that emitted light near the short wavelength end of the visible region (at ca. 400 nm). The next very low intensity emission band at 530 nm demonstrated that the ZnO material obtained is characterized by a low concentration of surface defects.

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Authors (7)

Kamila Sadowska dr hab. inż.
Joanna Karczewska-Golec
- University of Gdansk Department of Molecular Biology
Jakub Karczewski dr hab. inż.
Marcin Łoś
- University of Gdansk Department of Molecular Biology
Andrzej Kłonkowski prof. dr hab. inż.
- University of Gdansk Faculty of Chemistry
Grzegorz Węgrzyn
- University of Gdansk Department of Molecular Biology
Piotr Golec
- Laboratory of Molecular Biology (affiliated with the University of Gdansk) .

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: BIOCONJUGATE CHEMISTRY no. 27, edition 9, pages 1999 - 2006,
ISSN: 1043-1802
Language:: English
Publication year:: 2016
Bibliographic description:: Żelechowska K., Karczewska-Golec J., Karczewski J., Łoś M., Kłonkowski A., Węgrzyn G., Golec P.: Phage-Directed Synthesis of Photoluminescent Zinc Oxide Nanoparticles under Benign Conditions// BIOCONJUGATE CHEMISTRY. -Vol. 27, iss. 9 (2016), s.1999-2006
DOI:: Digital Object Identifier (open in new tab) 10.1021/acs.bioconjchem.6b00196
Verified by:: Gdańsk University of Technology