Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis

Karol Parchem; Shlomo Sasson; Carla Ferreri; Agnieszka Bartoszek-Pączkowska

doi:10.1080/10715762.2019.1657573

Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis

Abstract

Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes, but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterized by high nutritional value and other beneficial health effects, which are confirmed by numerous epidemiological studies. For this reason, PLs are of high interest in lipidomics that targets both the analysis of membrane lipid distribution as well as correlates composition of lipids with their effects on functioning of cells, tissues and organs. Lipidomic assessments follow-up the changes occurring in living organisms, such as free radical attack and oxidative modifications of the polyunsaturated fatty acids (PUFAs) build in PL structures. Oxidized PLs (oxPLs) can be generated exogenously and supplied to organisms with processed food or formed endogenously as a result of oxidative stress. Cellular and tissue oxPLs can be a biomarker predictive of the development of numerous diseases such as atherosclerosis or neuroinflammation. Therefore, suitable high-throughput analytical techniques, which enable comprehensive analysis of PL molecules in terms of the structure of hydrophilic group, fatty acid (FA) composition and oxidative modifications of FAs, have been currently developed. This review addresses all aspects of PL analysis, including lipid isolation, chromatographic separation of PL classes and species, as well as their detection. The bioinformatic tools that enable handling of large amount of data generated during lipidomic analysis are also discussed. In addition, imaging techniques such as confocal microscopy and mass spectrometry imaging for analysis of cellular lipid maps, including membrane PLs, are presented.

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Authors (4)

Karol Parchem dr inż.
Shlomo Sasson
- Faculty of Medicine, The Hebrew University of Jerusalem Institute for Drug Research
Carla Ferreri
- Consiglio Nazionale delle Ricerche Istituto per la SintesiOrganica e la Fotoreattivita
Agnieszka Bartoszek-Pączkowska prof. dr hab. inż.

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Articles

Type:

artykuły w czasopismach

Published in:

FREE RADICAL RESEARCH pages 1 - 33,
ISSN: 1071-5762

Language:

Polish

Publication year:

2019

Bibliographic description:

Parchem K., Sasson S., Ferreri C., Bartoszek-Pączkowska A.: Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis// FREE RADICAL RESEARCH -, (2019), s.1-33

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