Spatially variant PSF modeling in confocal macroscopy

Anna Węsierska; Hugues Talbot; Jean-Christophe Pesquet

doi:10.1109/isbi.2018.8363622

Spatially variant PSF modeling in confocal macroscopy

Abstract

The point spread function (PSF) of imaging systems plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view. In confocal macroscopy, the related artifacts are even stronger, as the field of view is much larger. Because the related PSFs are strongly spatially variant, it is essential to be able to model them with few parameters. The field aberrations in fluorescence macroscopy were observed to be symmetrical and to increase with the distance from the center of the field of view. In this paper we propose an experiment and an optimization method for assessing the center of the field of view. The obtained results constitute an important step towards reducing the number of parameters in macroscopy PSF models.

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Category:: Conference activity
Type:: materiały konferencyjne indeksowane w Web of Science
Language:: English
Publication year:: 2018
Bibliographic description:: Węsierska A., Talbot H., Pesquet J..: Spatially variant PSF modeling in confocal macroscopy, W: , 2018, ,.
DOI:: Digital Object Identifier (open in new tab) 10.1109/isbi.2018.8363622
Verified by:: Gdańsk University of Technology