Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

Katarzyna Grecka; Piotr Kuś; Randy Worobo; Piotr Szweda

doi:10.3390/molecules23020260

Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

Abstract

The antimicrobial activity of 144 samples of honeys including 95 products from apiaries located in Northern Poland was evaluated. The antibacterial activity of those natural products, their thermal stability, and activity in the presence of catalase was investigated by microdilution assays in titration plates. The MTT assay was performed for the determination of anti-biofilm activity. Spectrophotometric assays were used for the determination of antioxidant potential, total phenolic content, and ability to generate hydrogen peroxide. Some of the investigated honeys exhibited surprisingly high antimicrobial, especially anti-staphylococcal, potential, with Minimal Inhibitory Concentration (MIC) values of only 1.56% (v/v). Much higher resistance was observed in the case of staphylococci growing as biofilms. Lower concentrations of the product, up to 12.5% (v/v) stimulated its growth and effective eradication of biofilm required concentration of at least 25% (v/v). Hydrogen peroxide has been identified as a crucial contributor to the antimicrobial activity of honeys supplied by Polish beekeepers. However, some of the results suggest that phytochemicals, especially polyphenols, play an important role depending on botanical source (both positive, e.g., in the case of buckwheat honeys as well as negative, e.g., in the case of some rapeseed honeys) in their antimicrobial potential.

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Authors (4)

Katarzyna Grecka
Piotr Kuś dr
- Wydział Farmaceutyczny z O. Analityki Medycznej Katedra i Zakład Farmakognozji
Randy Worobo
- Department of Food Science
Piotr Szweda dr hab. inż.

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DOI:: Digital Object Identifier (open in new tab) 10.3390/molecules23020260
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Keywords

MIC

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: MOLECULES no. 23, edition 2, pages 1 - 23,
ISSN: 1420-3049
Language:: English
Publication year:: 2018
Bibliographic description:: Grecka K., Kuś P., Worobo R., Szweda P.: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland// MOLECULES. -Vol. 23, iss. 2 (2018), s.1-23
DOI:: Digital Object Identifier (open in new tab) 10.3390/molecules23020260
Verified by:: Gdańsk University of Technology