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Abstract
The enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as “structure breaking” solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we combine FTIR spectroscopy, DSC calorimetry and DFT calculations to gain an insight into the interactions between osmolytes and two proteins: lysozyme and ubiquitin. Despite high structural similarity, both osmolytes exert different influence on protein stability: taurine is a stabilizer, TMT is a denaturant. We show also that taurine amino group interacts directly with the side chains of proteins, whereas TMT does not interact with proteins at all. Although two solutes weaken on average the structure of the surrounding water, their hydration spheres are different. Taurine is surrounded by two populations of water molecules: bonded with weak H-bonds around sulfonate group, and strongly bonded around amino group. The strong hydrogen-bonded network of water molecules around the amino group of taurine further improves properties of enhanced protein hydration sphere and stabilizes the native protein form. Direct interactions of this group with surface side chains provide a proper orientation of taurine and prevents the SO3− group from negative influence. The weakened SO3− hydration sphere of TMT breaks up the hydrogen-bonded network of water around the protein and destabilizes it. However, TMT at low concentration stabilize both proteins to a small extent. This effect can be attributed to an actual osmophobic effect which is overcome if the concentration increases.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
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AMINO ACIDS
no. 50,
pages 125 - 140,
ISSN: 0939-4451 - Language:
- English
- Publication year:
- 2018
- Bibliographic description:
- Bruździak P., Panuszko A., Kaczkowska E., Piotrowski B., Daghir A., Demkowicz S., Stangret J.: Taurine as a water structure breaker and protein stabilizer// AMINO ACIDS. -Vol. 50, iss. 1 (2018), s.125-140
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s00726-017-2499-x
- Verified by:
- Gdańsk University of Technology
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