Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone

Aleksandra Królicka; Anna Szpitter; Krzysztof Stawujak; Rafał Barański; Anna Gwizdek-Wiśniewska; Anita Skrzypczak; Marian Antoni Kamiński; Ewa Łojkowska

doi:10.1007/s11240-010-9778-5

Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone

Abstract

Plants belonging to genus Drosera (family Droseraceae) contain pharmacologically active naphthoquinones such as ramentaceone and plumbagin. Hairy root cultures obtained following Agrobacterium rhizogenes-mediated transformation have been reported to produce elevated levels of secondary compounds as well as exhibit desirable rapid biomass accumulation in comparison to untransformed plants. The aim of this study was to establish hairy root or teratoma cultures of Drosera capensis var. alba and to increase the level of ramentaceone in transformed tissue by application of abiotic and biotic elicitors. The appearance of transformed tissues-teratomas but not hairy roots was observed 18 weeks after transformation. The transformation efficiency was 10% and all teratoma cultures displayed about 3 times higher growth rate than non-transformed cultures of D. capesis. The transformation was confirmed by PCR and Southern hybridization using primers based on the A. rhizogenes rolB and rolC gene sequences. HPLC analysis of ramentaceone content indicated 60% higher level of this metabolite in teratoma tissue in comparison to non-transformed cultures. Among the elicitors tested jasmonic acid (2.5 mg l−1) turned out to be the most effective. The productivity of ramentaceone in elicited teratoma cultures was about sevenfold higher than in liquid cultures of D. capensis var. alba and amounted to 2.264 and 0.321 mg respectively during 4 weeks of cultivation. This is the first report on the transformation of Drosera plant with A. rhizogenes.

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Authors (8)

Aleksandra Królicka
Anna Szpitter
Krzysztof Stawujak
Rafał Barański
Anna Gwizdek-Wiśniewska
Anita Skrzypczak
Marian Antoni Kamiński prof. dr hab. inż.
Ewa Łojkowska

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: PLANT CELL TISSUE AND ORGAN CULTURE no. 103, pages 285 - 292,
ISSN: 0167-6857
Language:: English
Publication year:: 2010
Bibliographic description:: Królicka A., Szpitter A., Stawujak K., Barański R., Gwizdek-Wiśniewska A., Skrzypczak A., Kamiński M., Łojkowska E.: Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone// PLANT CELL TISSUE AND ORGAN CULTURE. -Vol. 103, nr. iss. 3 (2010), s.285-292
DOI:: Digital Object Identifier (open in new tab) 10.1007/s11240-010-9778-5
Verified by:: Gdańsk University of Technology